Decomposition Study on Effects of Lianli Tang on Colon and Peripheral Blood Inflammatory Factors in TNBS-induced Rat Model

LIU Li-mei; ZHANG Qiu-hai; BAI Dong; ZHANG Ling; WANG Rui-hai; LIU Hong; LI Shu-li; DING Jia-xin; YUE Guang-xin

doi:10.13422/j.cnki.syfix.2014090152

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Decomposition Study on Effects of Lianli Tang on Colon and Peripheral Blood Inflammatory Factors in TNBS-induced Rat Model

更新时间：2024-01-04

- Decomposition Study on Effects of Lianli Tang on Colon and Peripheral Blood Inflammatory Factors in TNBS-induced Rat Model
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 20, Issue 9, Pages: 152-157(2014)
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- DOI：10.13422/j.cnki.syfix.2014090152
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- Published：2014
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LIU Li-mei, ZHANG Qiu-hai, BAI Dong, et al. Decomposition Study on Effects of Lianli Tang on Colon and Peripheral Blood Inflammatory Factors in TNBS-induced Rat Model[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(9): 152-157.
DOI：

LIU Li-mei, ZHANG Qiu-hai, BAI Dong, et al. Decomposition Study on Effects of Lianli Tang on Colon and Peripheral Blood Inflammatory Factors in TNBS-induced Rat Model[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(9): 152-157. DOI： 10.13422/j.cnki.syfix.2014090152.

摘要

目的：采用2，4，6-三硝基苯磺酸（TNBS）诱导的大鼠溃疡性结肠炎（UC）模型，观察连理汤各拆方药组及其配伍对UC大鼠外周血及结肠组织中细胞因子等含量的影响。方法：将连理汤拆分成补脾益肠药（A因素）、清热燥湿药（B因素）、温中散寒药（C因素），以含和不含该因素为1，2水平，采用正交设计表L8（27）设试验1~7组和UC组，增设柳氮磺吡啶组（SASP）与正常对照组。采用Sprague Dawley大鼠，前9组按TNBS乙醇灌肠法塑造UC模型，试验1~7组分别灌胃相应的拆方浸膏混悬液，剂量依次为1.02，1.47，0.98，1.24，0.29，0.145，0.087 g·kg-1，SASP组灌服柳氮磺吡啶（0.4 g·kg-1），以UC大鼠外周血及结肠组织中细胞因子等含量作为考察指标，观察灌服连理汤拆方14 d后各组对其含量的影响。结果：UC组大鼠结肠炎症环氧合酶-2（Cox-2）、白介素-1α（IL-1α）含量较正常对照组有显著升高（P<0.01），金属蛋白酶类组织抑制剂（tissue inhibitor of metalloproteinases 1，TIMP-1）和肿瘤坏死因子-α（tumor necrosis factor alpha，TNF-α）表达也有明显增强。补脾益肠药（A）在抑制结肠Cox-2方面影响显著（P<0.05）；各因素对结肠中IL-1α含量的影响均不显著，但全方效果显著（P<0.01）；补脾益肠药和清热燥湿药配伍（即A×B交互作用）对降低结肠TNF-α含量影响显著（P<0.05）；温中散寒药（C）以及补脾益肠药和温中散寒药配伍（即A×C交互作用）对抑制UC大鼠结肠组织中TIMP-1 含量有极显著影响（P<0.01），清热燥湿药（B）以及清热燥湿药和温中散寒药配伍（即B×C交互作用）对该指标也有显著影响（P<0.05）。UC大鼠血清中IL-1α，TNF-α明显下降，拆方各因素均无显著作用；UC大鼠血清TIMP-1明显升高，温中散寒药（C）对抑制UC模型大鼠外周血中TIMP 含量有显著性影响（P<0.05）。结论：补脾益肠药在连理汤的整体疗效中起主导作用，温中散寒药及其与温补药配伍也起重要作用。

Abstract

Objective: To carry out decomposition study on effects of Lianli Tang on colon and peripheral blood inflammatory factors in TNBS-induced rat model. Method: Lianli Tang were divided into three groups

tonify the spleen drugs (Panax Ginseng

Poria cocos

Atractylodes macrocephala koidz

Glycyrrhizae Radix et Rhizoma; symbol as A)

Warming the middle and dissipating cold drugs (Zingiberis Rhizoma;symbol as B)

clearing heat and drying dampness drugs (Coptidis Rhizoma; symbol as C)

as three factors to design the experiment using orthogonal design table L8 (27)

set up 1-7 group and UC group

then add sulfasalazine (SASP) and normal controls group (Control). Sprague Dawley rats were used to duplicate UC model by TNBS/ethanol solution through enema

1-7 group was administrated respectively by Lianli Tang decompositions through intragastric treatment

which the corresponding doses is 1.02

1.47

0.98

1.24

0.29

0.145

0.087 g·kg-1

SASP group rats were fed by sulfasalazine (0.4 g·kg-1). Cytokines of peripheral blood and colon were observed after rats were treated for 14 days. Result: Cyclooxygenase-2 (Cox-2)

interleukin-1α ( IL-1α) of UC rats were increased significantly than control (P<0.01)

tumor necrosis factor-α (TNF-α) expression were also significantly enhanced

A of Liali Tang significantly affect the effect of inhibiting colon Cox-2 (P<0.05)

each factors except the whole formula

have no effect in regulating colonic IL-1α

A×B significantly affected the results (P<0.05) in reducing colonic TNF-α

A×C (P<0.01) and B

B×C (P<0.05) in inhibiting colon tissue inhibitor of metall oproteimses 1 (TIMP-1) levels. Serum IL-1α

TNF-α of UC rats decreased apparently

each decomposition factor had no effect on the result;serum TIMP-1 was obviously increased

decomposition C show effect on inhibiting TIMP-1 (P<0.05). Conclusion: Tonify the spleen drugs played a major role in the overall effect of Lianli Tang

Warm the middle and dissipate cold drugs and its compatibility with tonify the spleen drugs also play an important role.

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