ZHOU Li-ya, ZOU Jia-hong, LI Xin. Effect of Shangzhongxia General Gout Decoction on Acute Gouty Arthritis and Th17 Cells[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(9): 199-202.
DOI:
ZHOU Li-ya, ZOU Jia-hong, LI Xin. Effect of Shangzhongxia General Gout Decoction on Acute Gouty Arthritis and Th17 Cells[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(9): 199-202. DOI: 10.13422/j.cnki.syfix.2014090199.
Effect of Shangzhongxia General Gout Decoction on Acute Gouty Arthritis and Th17 Cells
Objective: To study anti-inflammatory and analgesic effects of Shangzhongxia generic gout decoction (SGGD) on sodium urate induced acute gouty arthritis (GA). Method: 60 rats were randomly divided into six groups
the control group
the model group
indomethacin group
SGGD high dose group
middle dose group
low dose group (20
10
5 g·kg-1)
10 in each group. SGGD was given once each day for 7 days.1 h after the last administration
according to the classical method Coderre
in addition to the control group
and rats in the remaining five groups were injected ankle injection of sodium urate crystal solution to establish acute gouty arthritis model. The degree of joint swelling were measured the
Flow cytometry was used to detect the content of peripheral blood Th17 cells
Th17 cells (1.65±0.25) % was significantly lower than that in the model group (3.25±0.96) %. Th17 cell related inflammatory factor IL-17 (0.54±0.09) μg·L-1 was significantly lower than that in the model group (0.69±0.07) μg·L-1. Inflammatory IL-6 content was obviously lower than that in the model group (P<0.05). Conclusion: SGGD has better therapeutic effect on sodium urate gouty arthritis
the mechanism may be related to the decreasing Th17 cells and the level of inflammatory factors IL-17
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