YANG Lan~, WANG Man-yuan, ZHANG Dong, et al. Determination of Scopoletin in Qinghao by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2006, (10): 10-11.
YANG Lan~, WANG Man-yuan, ZHANG Dong, et al. Determination of Scopoletin in Qinghao by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2006, (10): 10-11. DOI: 10.13422/j.cnki.syfjx.2006.10.006.
Determination of Scopoletin in Qinghao by HPLC
摘要
目的:建立青蒿中东莨菪内酯的含量测定方法。方法:样品用甲醇超声提取;ODS柱
甲醇-0.08%磷酸(4∶6)为流动相
检测波长340nm。结果:东莨菪内酯获良好分离
在0.021
0
.42μg范围内呈现良好的线性关系(r=0.999 9)
加样回收率为99.3%
RSD为2.0%。结论:本法简单、准确、重复性好
可用于青蒿中东莨菪内酯的含量测定。
Abstract
Objective:To develop a method for the determination of scopoletin in Qinghao.Method:Sample was extracted with methanol.Scopoletin in Qinghao was determined by HPLC with ODS column.The mobile phase was methanol-0.08% H
3
PO
4
(4∶6)solution.The detection wavelength was 340nm.Result:Scopoletin in sample solution was well separated.Linearity of scopoletin was good(r=(0.999 9))in range of 0.021~0.42μg.The average recovery was 99.3%
RSD was 2.0%.Conclusion:The method is simple
accurate
repeatable and suitable for the determination of scopoletin in Qinghao.