Protection of （-）-Catechin Gallate and （+）-Epicatechin on Xanthine-Xanthine Oxidase System Injury in Cultured Cardiomyocytes

CAO Ying; LIANG Ri-xin~; YANG Bin; WANG Lan; YANG Qing; WANG Yan-li

doi:10.13422/j.cnki.syfjx.2006.10.017

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Protection of （-）-Catechin Gallate and （+）-Epicatechin on Xanthine-Xanthine Oxidase System Injury in Cultured Cardiomyocytes

更新时间：2024-09-23

- Protection of （-）-Catechin Gallate and （+）-Epicatechin on Xanthine-Xanthine Oxidase System Injury in Cultured Cardiomyocytes
- Chinese Journal of Experimental Traditional Medical Formulae Issue 10, Pages: 36-38(2006)
- 作者机构：
  
  1. 中国中医科学院中药研究所
  2. 中国中医科学院中药研究所北京100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2006.10.017
  CLC： R285.5
- Published：2006
- 稿件说明：
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[1]曹莹,梁日欣,杨滨,王岚,杨庆,王彦礼.(-)-儿茶素没食子酸酯和(+)-表儿茶素对心肌细胞保护作用研究[J].中国实验方剂学杂志,2006(10):36-38.

CAO Ying, LIANG Ri-xin~, YANG Bin, et al. Protection of （-）-Catechin Gallate and （+）-Epicatechin on Xanthine-Xanthine Oxidase System Injury in Cultured Cardiomyocytes[J]. Chinese journal of experimental traditional medical formulae, 2006, (10): 36-38.
[1]曹莹,梁日欣,杨滨,王岚,杨庆,王彦礼.(-)-儿茶素没食子酸酯和(+)-表儿茶素对心肌细胞保护作用研究[J].中国实验方剂学杂志,2006(10):36-38. DOI： 10.13422/j.cnki.syfjx.2006.10.017.

CAO Ying, LIANG Ri-xin~, YANG Bin, et al. Protection of （-）-Catechin Gallate and （+）-Epicatechin on Xanthine-Xanthine Oxidase System Injury in Cultured Cardiomyocytes[J]. Chinese journal of experimental traditional medical formulae, 2006, (10): 36-38. DOI： 10.13422/j.cnki.syfjx.2006.10.017.

摘要

目的:观察（-）-儿茶素没食子酸酯[（-）-CG]和（+）-表儿茶素[（+）-EC]对心肌细胞黄嘌呤/黄嘌呤氧化酶体系损伤的保护作用。方法:建立心肌细胞黄嘌呤/黄嘌呤氧化酶体系损伤模型

倒置显微镜观察加入（-）-CG、（+）-EC后细胞形态学变化;噻唑盐比色法测定细胞活性;测定细胞培养液中乳酸脱氢酶（LDH）、丙二醛（MDA）、超氧化物歧化酶（SOD）含量。结果:（-）-CG、（+）-EC能够增加心肌细胞存活率

降低心肌细胞LDH的漏出量

增加SOD活性

降低培养液中MDA的含量。结论:（-）-CG、（+）-EC具有抗体外培养大鼠乳鼠心肌细胞黄嘌呤/黄嘌呤氧化酶体系损伤的作用。

Abstract

Objective:To observe the protective effect of（-）-Catechin gallate and（+）-Epicatechin on Xanthine-Xanthine Oxidase System injury in cultured cardiomyocytes.Methods:The injury model of cultured neonatal rat cardiomyocytes was induced by Xanthine-Xanthine Oxidase System.Cell proliferation was detected by MTT assay

lactate dehydrogenase（LDH） activity

Maleic Dialdehyde（MDA） and Superoxide Dismutase（SOD） level in supernatant were measured with colorimetric assay.Results:Compared with model group

（-）-CG

（+）-EC can obviously increase survival rate of cardiomyocytes

decrease LDH activity and MDA level

increase SOD level in supernatant.Conclusion:（-）-CG、（+）-EC can protect cultured cardiomyocytes against Xanthine-Xanthine Oxidase System damage.

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