The Fingerprint for Adventitious Root of Radix Salviae Miltiorrhizae Developed by High-performance Liquid Chromatography and its Active Component Primary Analysis

WANG Xue-yong; CUI Guang-hong; HE Xi-rong; HUANG Lu-qi~

doi:10.13422/j.cnki.syfjx.2007.01.002

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The Fingerprint for Adventitious Root of Radix Salviae Miltiorrhizae Developed by High-performance Liquid Chromatography and its Active Component Primary Analysis

更新时间：2024-09-23

- The Fingerprint for Adventitious Root of Radix Salviae Miltiorrhizae Developed by High-performance Liquid Chromatography and its Active Component Primary Analysis
- Chinese Journal of Experimental Traditional Medical Formulae Issue 1, Pages: 1-4(2007)
- 作者机构：
  
  1. 中国中医科学院中药研究所
  2. 中国中医科学院中药研究所北京100700
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2007.01.002
  CLC： R284.1
- Published：2007
- 稿件说明：
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[1]王学勇,崔光红,何希荣,黄璐琦.建立高效液相指纹图谱分析无机离子对丹参不定根有效成分影响[J].中国实验方剂学杂志,2007(01):1-4.

WANG Xue-yong, CUI Guang-hong, HE Xi-rong, et al. The Fingerprint for Adventitious Root of Radix Salviae Miltiorrhizae Developed by High-performance Liquid Chromatography and its Active Component Primary Analysis[J]. Chinese journal of experimental traditional medical formulae, 2007, (1): 1-4.
[1]王学勇,崔光红,何希荣,黄璐琦.建立高效液相指纹图谱分析无机离子对丹参不定根有效成分影响[J].中国实验方剂学杂志,2007(01):1-4. DOI： 10.13422/j.cnki.syfjx.2007.01.002.

WANG Xue-yong, CUI Guang-hong, HE Xi-rong, et al. The Fingerprint for Adventitious Root of Radix Salviae Miltiorrhizae Developed by High-performance Liquid Chromatography and its Active Component Primary Analysis[J]. Chinese journal of experimental traditional medical formulae, 2007, (1): 1-4. DOI： 10.13422/j.cnki.syfjx.2007.01.002.

摘要

目的:建立丹参不定根RP-HPLC指纹图谱分析方法

分析无机离子对丹参不定根影响。方法:高效液相色谱法

RP-Waters C18（150 mm×3.9 mm

5μm）

流动相为0.5%醋酸甲醇和0.5醋酸%水梯度洗脱

流速1.0 mL.min-1

检测波长:270nm

柱温:30℃。对丹参不定根进行指纹图谱分析。结果:在此色谱条件下

3组共9份样品的不定根可检测出11个相对位置稳定的共有峰

其中确定9个共有峰作为成分变化指示峰

并确定了5个已知成分

结果显示无机金属离子能明显提高不定根中丹酚酸类成分的含量

而对丹参酮等脂溶性成分影响不显著。结论:该方法稳定可靠

重复性强

可用于评价对丹参不定根次生代谢成分变化情况

为丹参不定根的培养及进一步研究奠定基础。

Abstract

Objective:To establish RP-HPLC fingerprint for adventitious root of Radix Salviae Miltiorrhizae and primarily investigate effects of different mental ion on it.Method:The Methanol extraction of Radix Salviae Miltiorrhizae was filtered through 0.45 mm filters and separated by using a Resolve TM C18 column（Waters

150mm×3.9 mm

5 μm） at 30℃.The mobile phase consisted of acetonitrile and water was used as gradient elution for the separation.The flow rate was set at 1.0 mL·min

-1

and detection was set at 270 nm.The fingerprint was then established as above condition for adventitious root of Radix Salviae Miltiorrhizae Result:11 peaks in common of 9 samples of treated with 3 kind of metal ion to adventitious root were acquired and 9 of them were chosen as symbolization for the component of Radix Salviae Miltiorrhizae.Conclution:The fingerprint profile provides a good approach to value the quality and monitor the change of content of active component in adventitious root of Radix Salviae Miltiorrhizae.

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Related Author

WANG Xue-yong

CUI Guang-hong

HE Xi-rong

HUANG Lu-qi

ZHU Zhao-jing1

DENG Kai-ying2

ZHENG Xiao-ke

DONG San-li

Related Institution

Henan College of Traditional Chinese Medicine

ZHEJIANG A & F UNIVERSITY

Nanjing University of Traditional Chinese Medicine,Key Laboratory for Modern Research of Traditional Chinese Medical Formulae of Jiangsu

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

College of Pharmacy,Yanbian University,Yanji

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