Simultaneous Determination of Five Main Constituents in Xianlingpi Granules by HPLC

CHEN Yan~1; CAI Yin~2; JIA Xiao-bin~2; DING An-wei~

doi:10.13422/j.cnki.syfjx.2007.11.002

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Simultaneous Determination of Five Main Constituents in Xianlingpi Granules by HPLC

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- Simultaneous Determination of Five Main Constituents in Xianlingpi Granules by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Issue 11, Pages: 1-3(2007)
- 作者机构：
  
  1. 南京中医药大学
  2. 江苏省中医药研究院江苏省现代中药制剂工程技术研究中心
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2007.11.002
  CLC： R286
- Published：2007
- 稿件说明：
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[1]陈彦,蔡垠,贾晓斌,丁安伟.高效液相色谱法测定仙灵脾配方颗粒中5种主要黄酮类成分的含量[J].中国实验方剂学杂志,2007(11):1-3.

CHEN Yan~1, CAI Yin~2, JIA Xiao-bin~2, et al. Simultaneous Determination of Five Main Constituents in Xianlingpi Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2007, (11): 1-3.
[1]陈彦,蔡垠,贾晓斌,丁安伟.高效液相色谱法测定仙灵脾配方颗粒中5种主要黄酮类成分的含量[J].中国实验方剂学杂志,2007(11):1-3. DOI： 10.13422/j.cnki.syfjx.2007.11.002.

CHEN Yan~1, CAI Yin~2, JIA Xiao-bin~2, et al. Simultaneous Determination of Five Main Constituents in Xianlingpi Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2007, (11): 1-3. DOI： 10.13422/j.cnki.syfjx.2007.11.002.

摘要

目的:建立反相高效液相色谱法同时测定仙灵脾配方颗粒中5种主要黄酮类成分:淫羊藿苷、朝藿定A、朝藿定B、朝藿定C、宝藿苷I的含量。方法:采用RP-HPLC

色谱柱ZORBAX SB-C18（4.6 mm×250 mm

5μm）;流动相:乙腈-水;梯度洗脱程序:（0

5）min乙腈10%

（15

8）min乙腈25%

（38

1）min乙腈25%

1%;流速1 mL.min-1;检测波长270nm;柱温:30℃。结果:5种成分均达到基线分离

线性良好。结论:该方法快速、准确、重复性好

为仙灵脾配方颗粒的质量控制提供了理论依据。

Abstract

Objective:To develop a HPLC method of simultaneous determination of Icarrin

Epimedin A

Epimedin B

Epimedin C

Baohuoside I in Xianlingpi granules.Method:The samples were separated at 30℃ on a ZORBAX SB-C

column（4.6 mm×250 mm

5 μm） eluted with acetonitrile and water in gradient mode.The ratio of acetonitrile increased from 10% to 25% in 15 min

and kept constant for 23 min

then from 25% to 71% during 23 min.The detection wavelength was 270 nm

and the flow rate was(1 mL·min

-1

).Result:Five compounds were baseline-isolated

the linear relationships of lcarrin

Epimedin A

Epimedin B

Epimedin C

Baohuosiede I were excellent.Conclusion:The method was rapid and precise

and can be use for controlling the quality of Xianlingpi granules.

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Related Author

CHEN Yan1

CAI Yin2

JIA Xiao-bin2

DING An-wei1

ZOU Sheng-qin

HUANG Hao

YU Meng

LIU Wen-qiao

Related Institution

Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine（TCM），School of Pharmacy，Henan University of Chinese Medicine

Luohe Medical College

Third Affiliated Hospital of Henan University of Chinese Medicine

Tianjin University of Traditional Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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