FAN Min-wei, MA Neng-yi, WANG Bing~, et al. Determination of hyperin in sustained-release capsules of Hypericum perforatum extract by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2007, (11): 9-11.
FAN Min-wei, MA Neng-yi, WANG Bing~, et al. Determination of hyperin in sustained-release capsules of Hypericum perforatum extract by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2007, (11): 9-11. DOI: 10.13422/j.cnki.syfjx.2007.11.005.
Determination of hyperin in sustained-release capsules of Hypericum perforatum extract by HPLC
Objective:To develop an HPLC method for the determination of hyperin in Hypericum perforatum L.extract sustained-release capsules.Methods:The analysis was performed on an Agilent ZORBAX SB-C
18
(5 μm
4.5 mm×150 mm) column eluted with the mobile phase consisted of water-acetonitrile-phosphoric acid(825∶175∶1).The detection wavelength was set at 368 nm.Result:The linear range for hyperin was(0.06~0.30)μg(r=(0.999 9).)Conclusion:This method can be used effectively for the quality control of the capsules.It was simple