XIE Dong~. Determination of Indirubin in Shengxuexiaoban Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2008, (1): 4-5.
XIE Dong~. Determination of Indirubin in Shengxuexiaoban Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2008, (1): 4-5. DOI: 10.13422/j.cnki.syfjx.2008.01.010.
Determination of Indirubin in Shengxuexiaoban Tablets by HPLC
摘要
目的:应用高效液相色谱法测定升血小板片中靛玉红的含量
控制该制剂的质量。方法:采用HPLC法
色谱柱为Kromasil C18柱(4.6 mm×250 mm
5μm)
流动相为乙腈-水(53:47)
检测波长290 nm。结果:靛玉红线性范围(0.3028
3
.028)μg
平均加样回收率为97.4%
RSD=0.7%(n=6)。结论:该法操作简便易行、准确
可用于升血小板片的质量控制。
Abstract
Objective:To develop a HPLC method for determination of indirubin in Shengxuexiaoban Tablets.Methods:Kromasil C
18
(4.6 mm×250 mm
5 μm) column was used with acetonitrile-water(53∶47) as mobile phase
and the detection wavelength was set at 290 nm.Results: The linear range was(0.3028~3.028)μg for indirubin(r=(0.999 7))
the average recovery was 97.4% and RSD was 0.7%(n=6).Conclusions:The method is simple
reliable
and accurate for the determination of indirubin in this preparation.