Determination of Chlorogenic Acid in Stem bark and Leaf of Acanthopanacis Giraldii from Different Collection Time by RP-HPLC

SUN Zhuo-ran; LIU Yuan~; MENG Qing-yan; LI Ying; LIU Chao

doi:10.13422/j.cnki.syfjx.2009.02.006

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Determination of Chlorogenic Acid in Stem bark and Leaf of Acanthopanacis Giraldii from Different Collection Time by RP-HPLC

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- Determination of Chlorogenic Acid in Stem bark and Leaf of Acanthopanacis Giraldii from Different Collection Time by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 15, Issue 2, Pages: 15-17(2009)
- 作者机构：
  
  西南民族大学少数民族药物研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2009.02.006
  CLC： R284.1
- Published：2009
- 稿件说明：
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[1]孙卓然,刘圆,孟庆艳,李莹,刘超.RP-HPLC测定四川产红毛五加药材茎皮及叶中不同采收期绿原酸的含量[J].中国实验方剂学杂志,2009,15(02):15-17.

SUN Zhuo-ran, LIU Yuan~, MENG Qing-yan, et al. Determination of Chlorogenic Acid in Stem bark and Leaf of Acanthopanacis Giraldii from Different Collection Time by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(2): 15-17.
[1]孙卓然,刘圆,孟庆艳,李莹,刘超.RP-HPLC测定四川产红毛五加药材茎皮及叶中不同采收期绿原酸的含量[J].中国实验方剂学杂志,2009,15(02):15-17. DOI： 10.13422/j.cnki.syfjx.2009.02.006.

SUN Zhuo-ran, LIU Yuan~, MENG Qing-yan, et al. Determination of Chlorogenic Acid in Stem bark and Leaf of Acanthopanacis Giraldii from Different Collection Time by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(2): 15-17. DOI： 10.13422/j.cnki.syfjx.2009.02.006.

摘要

目的:分析四川产红毛五加药材中不同采收期茎皮及叶中绿原酸含量的差异。方法:测定四川产红毛五加茎皮药材及叶中不同采收期绿原酸的含量。色谱条件:KromasilC18柱（4.6 mm×250 mm

5μm）

柱温30℃。流动相乙腈-2‰磷酸水溶液（10∶90）

检测波长327 nm

流速1 mL.min-1。结果:绿原酸在0.355

.55μg与峰面积具有良好的线性关系;回归方程为Y=2.11×106X-2.24×105

r=0.999 5（n=6）;平均回收率为100.1%

RSD为0.9%（n=9）。结果:红毛五加茎皮药材花期（6

）月绿原酸含量较低

其苗期（3

月）、果期（8

月）的含量均保持在比较高的水平;同采收时间的叶中绿原酸高于茎皮。结论:从成分绿原酸的角度考虑

建议川产红毛五加叶可作为绿原酸提取新的源料

茎皮药材不在花期（6

月）采收。

Abstract

Objective:To determine the chlorogenic acid in stem bark and leaf of Acanthopanacis giraldii from different collection time. Methods: Chromatography parameter: kromasil C

（4.6 mm×250 mm

5 μm）;column temperature:30 ℃;the mobile phase: acetonitrile-（2 ‰） and phosphoric acid water（10∶90） aqueous solution;detection wavelength: 327 nm;flow rate:1 mL·min

-1

.Results: The method showed good linearity within the concentration ranges of 0.355～3.55 μg（r=（0.999 5））.The average recovery was 100.1%.The contents in the anthesis（from June to July）were lower

and the contents in the fructescence（from August to September） were higher and the content in leaf was higher than that in the bark.Conclusion: This method is simple

accurate

roducible and suitable for the determination of chlorogenic acid in stem bark and leaf of Acanthopanacis giraldii.Giving the consideration of chlorogenic acid

we suggested that the leaf of Acanthopanacis giraldii Harms can be used a new recourse of chlorogenic acid and the best collection time of Cortex Acanthopanacis giraldii were in the frutescence（August～Desember）.

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Related Author

SUN Zhuo-ran

LIU Yuan

MENG Qing-yan

LI Ying

LIU Chao

WANG Ling-ling

WANG Ling

YANG Fei

Related Institution

Ethnic Pharmaceutical Institute of Southwest University for Nationalities

Institute of Traditional Chinese Medicine（TCM），Shanghai University of TCM

Yanling DU Renkang Biotechnology Co. Ltd.

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Henan University of Chinese Medicine

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