YU Cui-cui, JIN Xiang-qun~, YUE Jun-wei, et al. Quantitative Determination of Cinobufagin and Resibufogenin in Bangqinmanyan Drop Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(2): 17-19.
YU Cui-cui, JIN Xiang-qun~, YUE Jun-wei, et al. Quantitative Determination of Cinobufagin and Resibufogenin in Bangqinmanyan Drop Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(2): 17-19. DOI: 10.13422/j.cnki.syfjx.2009.02.007.
Quantitative Determination of Cinobufagin and Resibufogenin in Bangqinmanyan Drop Pills by HPLC
摘要
目的:建立用HPLC测定蒡芩慢咽滴丸中蟾酥内酯含量的方法。方法:采用十八烷基键合硅胶色谱柱(250 mm×4.6 mm
Objective:To develop an HPLC method for quantitative determining of cinobufagin and resibufogenin in Bangqinmanyan Drop Pills.Methods: Diamonsil C
18
column(250 mm×4.6 mm
5 μm) was used as the stationary phase
the mixfure of acetonitrile-KH
2
PO
4
(the pH of solution was adjusted to 3.2 by phosphate acid)(32∶68) was served as mobile phase.The wavelength for detection was 296 nm
the column temperature was 40 ℃.Results:The linearity range were 0.2~1.0 μg(r=(0.999 7)) and 0.224~1.12 μg(r=(0.999 6)).The average recovery were 99.91%(RSD=(0.796 0%)) and 99.84(RSD=(1.002 3%)).Conclusion: The method is simple and reliable.