Effect of Gengnianchun Decoction on Bone Metabolism-Related Genes Expression in bone marrow microenvironment

YANG Xiao-li~1; LI Bin~1; LI Da-jin~1; JIN Wei-fang~2; ZHOU Wen-jiang~3; WANG Wen-jun~

doi:10.13422/j.cnki.syfjx.2009.02.011

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Effect of Gengnianchun Decoction on Bone Metabolism-Related Genes Expression in bone marrow microenvironment

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- Effect of Gengnianchun Decoction on Bone Metabolism-Related Genes Expression in bone marrow microenvironment
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 15, Issue 2, Pages: 26-29(2009)
- 作者机构：
  
  1. 复旦大学附属妇产科医院
  2. 复旦大学放射医学研究所
  3. 复旦大学实验动物部
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2009.02.011
  CLC： R285.5
- Published：2009
- 稿件说明：
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[1]杨晓莉,李斌,李大金,金慰芳,周文江,王文君.更年春方对骨代谢相关基因表达的影响[J].中国实验方剂学杂志,2009,15(02):26-29.

YANG Xiao-li~1, LI Bin~1, LI Da-jin~1, et al. Effect of Gengnianchun Decoction on Bone Metabolism-Related Genes Expression in bone marrow microenvironment[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(2): 26-29.
[1]杨晓莉,李斌,李大金,金慰芳,周文江,王文君.更年春方对骨代谢相关基因表达的影响[J].中国实验方剂学杂志,2009,15(02):26-29. DOI： 10.13422/j.cnki.syfjx.2009.02.011.

YANG Xiao-li~1, LI Bin~1, LI Da-jin~1, et al. Effect of Gengnianchun Decoction on Bone Metabolism-Related Genes Expression in bone marrow microenvironment[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(2): 26-29. DOI： 10.13422/j.cnki.syfjx.2009.02.011.

摘要

目的:观察更年春方对去卵巢（OVX）大鼠骨髓微环境中骨保护素（OPG）、破骨细胞分化因子（RANKL）和巨噬细胞集落刺激因子（M-CSF）mRNA表达的影响

探讨更年春抗绝经后骨质疏松骨丢失的可能分子机制。方法:取健康10月龄雌性SD大鼠42只

随机平均分为假手术组（S）

生理盐水组（N）、尼尔雌醇组（E）、更年春高剂量组（H）、更年春中剂量组（M）和更年春低剂量组（L）。假手术组仅切除卵巢周围少许脂肪

其余5组均切除卵巢。灌药3d后取腰椎冲洗骨髓提取总RNA

实时定量PCR法检测上述因子mRNA的表达情况。结果:更年春高剂量组可上调OVX大鼠骨组织中OPG的mRNA的表达

下调M-CSF的mRNA的表达

使RANKL/OPG值下降。结论:更年春方抗绝经后骨质疏松骨丢失的分子机制可能与其调控OPG和M-CSF的表达

影响RANKL/OPG值有关。

Abstract

Objiective:To observe the effect of Gengnianchun Decoction（GNC） on the mRNA expression of osteoprotegerin（OPG）

osteoclast differentiation factor（RANKL） and macrophage colony stimulating factor（M-CSF） in bone marrow microenvironment of ovariectomized rats

and investigate the possible pathway of GNC in preventing and treating postmenopausal osteoporosis.Methods:Forty-two healthy adult SD rats were randomly divided into sham operation group（S group）

saline group（N group）

Different concentrations of GNC groups: high

moderate and low level groups（H

and L group）and nilestriol group（E group）.All rats were ovariectomized except those in the sham operation group.After feding with medicines for three days

the total RNA was extracted from the lumbar vertebra to examine mRNA expression of RANKL

OPG and M-CSF by real-time PCR.Results:H group up-regulated the expression of OPG of the ovarietomized rat

whereas down-regulated the expression of M-CSF and decreased RANKL∶OPG ratio.Conclusion:The effect of GNC in treating postmenopausal osteoporosis is closely correlated with the regulation of OPG and M-CSF expressions and RANKL/OPG.

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Related Author

WANG Wen-jun1

ZHOU Wen-jiang3

JIN Wei-fang2

LI Da-jin1

LI Bin1

YANG Xiao-li1

Lin Na

CHEN Wei-heng

Related Institution

College of Traditional Chinese Medicine,Chongqing Medical University

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