SHAN Li-mei~1, ZHAO Yan-ling~. Establishment of the Rat Gastric Hot Model[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(2): 30-32.
SHAN Li-mei~1, ZHAO Yan-ling~. Establishment of the Rat Gastric Hot Model[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(2): 30-32. DOI: 10.13422/j.cnki.syfjx.2009.02.028.
Objective: To establish rat gastric hot model for relevant Chinses medicine pharmacological study.Methods: The rat gastric hot model was established by oral administration with red pepper and alcohol mixture for two weeks and irritated for three days by clipping tails.The temperature
amount of food
water
urination and defecation of every rats were detected by the help of metabolism cage.The content of IL-8
IL2
INF-α
TXB
2
6-keto-PGF1α in blood of rat was examined.Meanwhile
the injury extent of gastric mucosa and the histological characteristics of gastric mucosa were observed.Results: Two weeks after modeling
the model rats had the symptoms of temperature
weight and defecation decreecing and thirst for drinking.Congestion in gastric mucosa occurred and the blood level of IL8
IL-2
(INF-α
)TXB
2
6-keto-PGF1α were higher than those in the control group.However
in model rats pretreated with Zuojin pills
the symptoms and signs of hot-syndrome and histological changes were relieved.Conclusion: Under the controlled condition
feeding rats with red pepper and alcohol mixture combined with clipping tails can be successfully used to establish rat gastric model of hot-syndrome.
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Related Author
SHAN Li-mei1
ZHAO Yan-ling1
KONG Wei-jun1
HONG Wei2
LIU Zhi-guo2
XIAO Xiao-he1
CHEN Yuhang
ZHANG Ye
Related Institution
Dongfang Hospital, Beijing University of Chinese Medicine
Yunnan Provincial Hospital of Traditional Chinese Medicine
School of Clinical Medicine,Beijing University of Chinese Medicine
Beijing Key Laboratory of Chinese Materia Pharmacology,National Clinical Research Center of Traditional Chinese Medicine for Cardiovascular Diseases,Institute of Basic Medical Sciences, Xiyuan Hospital,China Academy of Chinese Medical Sciences