XUE Xiao-ping, LU Yan-min, WANG Qian, et al. Determination of Aloe-emodin,Rhein,Emodin,Chrysophanol and Rheochrysidin in QING RE JIE DU FANG by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(7): 6-8.
XUE Xiao-ping, LU Yan-min, WANG Qian, et al. Determination of Aloe-emodin,Rhein,Emodin,Chrysophanol and Rheochrysidin in QING RE JIE DU FANG by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(7): 6-8. DOI: 10.13422/j.cnki.syfjx.2009.07.014.
Determination of Aloe-emodin,Rhein,Emodin,Chrysophanol and Rheochrysidin in QING RE JIE DU FANG by HPLC
Objective:To develop an HPLC method for determination of aloe-emodin
rhein
emodin
chrysophanol and physcion in QING RE JIE DU entract.Methods: The analysis was carried out on a Kromasil C
1
8(250 mm×4.6 mm
5 μm)column eluted with a mobile phase of methanal-water containing 0.2% phosphoric acid solution(V∶V=85∶15).The flow rate was 1.0 mL·min-1 and column temperature was 35 ℃.The detection wavelength was set at 254 nm.Results: The calibration curve was linear within the range of 1.012 5~16.2
1.025~16.4
1.025~16.4
1.025~16.4 and 0.625~10 μg for aloe-emodin
rhein
emodin
chrysophanol and physcion
respectively.The average recoveries were 101.5%
99.2%
101.9%
101.5%
101.4% for the abore five conponents
respectively.Conclusion: This method is convenient
accurate and reproducible for determination of aloe-emodin
rhein
emodin
chrysophanol and phascion in QING RE JIE DU extraet.
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Related Author
XUE Xiao-ping
LU Yan-min
WANG Qian
LIU Hong-bin
WANG Qiang
QIAN Chun-hua
SUN Zhi-ming
WANG Jin-jin
Related Institution
Zhejiang Institute for Food and Drug Contrl
Zhejiang Chinese Medical University
Key Laboratory of Digital Quality Evaluation Technology of Chinese Materia Medica, State Administration of Traditional Chinese Medicine(TCM), Engineering Technology Research Center for Chinese Materia Medica Quality of Universities in Guangdong Province, School of TCM, Guangdong Pharmaceutical University
Xuzhou Medical Colledge
School of Chinese Materia Medica, Beijing University of Chinese Medicine