Determination of Ginsenoside Rg1、Re 、Rb1 and Notoginsenoside R1 in Lingzhi Jiangtang Capsule by HPLC

LI Yu-cui; CHEN Zhi-wei; BAO Mei-jia; HU Jia-min; PENG Shao-zhong; LI Ming; SU Zi-ren

doi:10.13422/j.cnki.syfjx.2009.07.015

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Determination of Ginsenoside Rg₁、Re 、Rb₁ and Notoginsenoside R₁ in Lingzhi Jiangtang Capsule by HPLC

更新时间：2024-09-23

- Determination of Ginsenoside Rg₁、Re 、Rb₁ and Notoginsenoside R₁ in Lingzhi Jiangtang Capsule by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 15, Issue 7, Pages: 9-11(2009)
- 作者机构：
  
  广州中医药大学
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2009.07.015
  CLC： R286
- Published：2009
- 稿件说明：
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[1]黎玉翠,陈志维,暴梅佳,胡家敏,彭绍忠,李明,苏子仁.HPLC法测定灵芝降糖胶囊中人参皂苷Rg_1、Re、Rb_1及三七皂苷R_1的含量[J].中国实验方剂学杂志,2009,15(07):9-11.

LI Yu-cui, CHEN Zhi-wei, BAO Mei-jia, et al. Determination of Ginsenoside Rg₁、Re 、Rb₁ and Notoginsenoside R₁ in Lingzhi Jiangtang Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(7): 9-11.
[1]黎玉翠,陈志维,暴梅佳,胡家敏,彭绍忠,李明,苏子仁.HPLC法测定灵芝降糖胶囊中人参皂苷Rg_1、Re、Rb_1及三七皂苷R_1的含量[J].中国实验方剂学杂志,2009,15(07):9-11. DOI： 10.13422/j.cnki.syfjx.2009.07.015.

LI Yu-cui, CHEN Zhi-wei, BAO Mei-jia, et al. Determination of Ginsenoside Rg₁、Re 、Rb₁ and Notoginsenoside R₁ in Lingzhi Jiangtang Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(7): 9-11. DOI： 10.13422/j.cnki.syfjx.2009.07.015.

摘要

目的:建立灵芝降糖胶囊的含量测定方法。方法:采用高效液相色谱法对制剂中的人参皂苷Rg1、Re、Rb1及三七皂苷R1进行定量测定。结果:人参皂苷Rg1在3.86～23.16μg范围内线性关系良好

r=0.999 9

平均回收率100.18%

RSD=1.52%;人参皂苷Re在0.628～3.768μg范围内线性关系良好

r=0.999 9

平均回收率100.22%

RSD=0.41%;人参皂苷Rb1在3.74～22.44μg范围内线性关系良好

r=0.999 9

平均回收率100.51%

RSD=0.74%;三七皂苷R1在0.764～4.584μg范围内线性关系良好

r=0.999 9

平均回收率99.05%

RSD=1.10%。结论:所建立的方法简便、快捷

重复性好

可用于该制剂的质量控制。

Abstract

Objective:To establish a method for the determination of effective components in Lingzhi Jiangtang Capsule.Methods: The contents of ginsenoside Rg

、Re、Rb

and notoginsenoside R

in Lingzhi Jiangtang Capsule were determined by HPLC.Results: The resolutions of the determined peaks were fine.The linearity ranges for ginsenoside Rg

ginsenoside Re

ginsenoside Rb

and notoginsenoside R

were 3.86～23.16 μg（r=0.999 9）

0.628～3.768 μg（r=0.999 9）

3.74～22.44 μg（r=0.999 9） and 0.764～4.584 μg（r=0.999 9） respectively.The average recoveries were 100.18% with RSD 1.52%

100.22% with RSD 0.41%

100.51% with RSD 0.74%

and 99.05% with RSD 1.10% for ginsenoside Rg

ginsenoside Re

ginsenoside Rb

notoginsenoside R

respectively.Conclusion: The quantitative method for determining the ingredient of Lingzhi Jiangtang Capsule is simple

feasible and repeatable

and is beneficial to quality control of Lingzhi Jiangtang Capsule.

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Related Author

LI Yu-cui

CHEN Zhi-wei

BAO Mei-jia

HU Jia-min

PENG Shao-zhong

LI Ming

SU Zi-ren

ZHANG Rong1

Related Institution

Guangzhou University of Chinese Medicine

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School of Chinese Materia Medica,Beijing University of Traditional Chinese Medicine

College of Traditional Chinese Medicine,Hebei United University

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