HPLC Determination of Saponins in Zhixiao Suppositories

ZHANG Rong1; Deng Xiu-xia2; WEI Gang1; LI Run-mei1; LI Wen-zhou1; CHEN Jun1

doi:10.13422/j.cnki.syfjx.2009.07.021

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HPLC Determination of Saponins in Zhixiao Suppositories

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- HPLC Determination of Saponins in Zhixiao Suppositories
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 15, Issue 7, Pages: 25-27(2009)
- 作者机构：
  
  1. 广州中医药大学
  2. 广东轻工职业技术学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2009.07.021
  CLC： R286
- Published：2009
- 稿件说明：
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[1]张荣,邓秀霞,卫罡,李润美,李文周,陈军.痔消栓的HPLC含量测定[J].中国实验方剂学杂志,2009,15(07):25-27.

ZHANG Rong1, Deng Xiu-xia2, WEI Gang1, et al. HPLC Determination of Saponins in Zhixiao Suppositories[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(7): 25-27.
[1]张荣,邓秀霞,卫罡,李润美,李文周,陈军.痔消栓的HPLC含量测定[J].中国实验方剂学杂志,2009,15(07):25-27. DOI： 10.13422/j.cnki.syfjx.2009.07.021.

ZHANG Rong1, Deng Xiu-xia2, WEI Gang1, et al. HPLC Determination of Saponins in Zhixiao Suppositories[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(7): 25-27. DOI： 10.13422/j.cnki.syfjx.2009.07.021.

摘要

目的:建立痔消栓的HPLC含量测定方法。方法:采用HPLC法

以Kromasil C18（4.6 mm×250 mm

5μm）色谱柱为固定相

以乙腈-0.2%磷酸水溶液为流动相

梯度洗脱

流速1.0 mL.min-1

柱温23℃

检测波长为203 nm。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1分别在0.368～2.76μg

0.676～5.07μg

0.812～6.09μg范围内线性关系良好

Abstract

Objective: To denvolop HPLC an method for the determination of the content of three saponins in Zhixiao suppositories.Methods: Analysis carriedout was performed on a Kromasil C

8 column（4.6 mm×250 mm.

5 μm）at 23 ℃.The mobile phases were methanol and 0.2% phosphoricacid solution in a gradient elution.The flow rate was set at 1.0 mL·min-1 and the ultraviolet detector was operated at 203 nm.Results: The linear ranges of notoginsenoside R

ginsenoside Rg

and ginsenoside Rb

were 0.368～2.76 μg

0.676～5.07 μg and 0.812～6.09 μg

respectively.The average recoveries of the three saponins were 98.53%

99.20% and 99.35%

respectively.The RSD（n=5）were 2.11%

1.88% and 2.38%

respectively.Conclusion: The separation is a good and the results are accurate and reproducible.It can be used for the quality control of Zhixiao suppositories.

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Related Author

ZHANG Rong1

Deng Xiu-xia2

WEI Gang1

LI Run-mei1

LI Wen-zhou1

CHEN Jun1

LI Yu-cui

CHEN Zhi-wei

Related Institution

Guangzhou University of Chinese Medicine

Guangdong Institute of Traditional Chinese Medicine

School of Pharmaceutical Science,Shanxi Medical University

Shanxi Province Chinese Medicine Modern Engineering Technology Research Center

School of Chinese Materia Medica,Beijing University of Traditional Chinese Medicine

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