Effect of Busuishengxue Granule on CAA c-kit mRNA and CD117 of Bone Marrow Mononuclear Cells

ZHANG Shou-lin~1; QI De-jie~2; SUN Wei-zheng~

doi:10.13422/j.cnki.syfjx.2009.09.004

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Effect of Busuishengxue Granule on CAA c-kit mRNA and CD117 of Bone Marrow Mononuclear Cells

更新时间：2024-09-23

- Effect of Busuishengxue Granule on CAA c-kit mRNA and CD117 of Bone Marrow Mononuclear Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 15, Issue 9, Pages: 49-51(2009)
- 作者机构：
  
  1. 长春中医药大学附属医院
  2. 吉林省通榆县第一人民医院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2009.09.004
  CLC： R259
- Published：2009
- 稿件说明：
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[1]张守琳,齐德杰,孙伟正.补髓生血颗粒对慢性再生障碍性贫血患者骨髓单个核细胞c-kit mRNA及CD117影响的研究[J].中国实验方剂学杂志,2009,15(09):49-51.

ZHANG Shou-lin~1, QI De-jie~2, SUN Wei-zheng~. Effect of Busuishengxue Granule on CAA c-kit mRNA and CD117 of Bone Marrow Mononuclear Cells[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(9): 49-51.
[1]张守琳,齐德杰,孙伟正.补髓生血颗粒对慢性再生障碍性贫血患者骨髓单个核细胞c-kit mRNA及CD117影响的研究[J].中国实验方剂学杂志,2009,15(09):49-51. DOI： 10.13422/j.cnki.syfjx.2009.09.004.

ZHANG Shou-lin~1, QI De-jie~2, SUN Wei-zheng~. Effect of Busuishengxue Granule on CAA c-kit mRNA and CD117 of Bone Marrow Mononuclear Cells[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(9): 49-51. DOI： 10.13422/j.cnki.syfjx.2009.09.004.

摘要

目的:探讨补髓生血颗粒对慢性再生障碍性贫血（CAA）患者骨髓单个核细胞c-kit mRNA及CD117表达的影响。方法:分别采用逆转录-聚合酶链反应（RT-PCR）法及流式细胞技术检测CAA病人治疗前后骨髓单个核细胞c-kit mRNA及CD117水平的变化。结果:CAA患者骨髓c-kit mRNA及其CD117表达水平高于正常对照组

治疗后二者表达水平均有不同程度下降

并且补髓生血颗粒对二者表达水平的下调程度优于再障生血片对照组（P<0.05）。结论:CAA患者骨髓c-kit mRNA及CD117的表达存在异常;补髓生血颗粒剂可能通过调节骨髓c-kit mRNA及CD117的表达来改善CAA的骨髓造血功能

促进骨髓造血功能恢复。

Abstract

Objective:To discuss the clinical effective of Busuishengxue granules to chronic aplastic anemia（CAA） patients

and to observe the difference in expression level of myelo-cytokine c-kit/CD117 before and after treatment.Methods:This experiment employed reverse transcription-polymerase chain reaction （RT-PCR） method to investigate the change of expression level of bone marrow mononuclear cell（BMMC） c-kit receptor mRNA and its c-kit receptor protein CD117 with flow cytometer method（FCM）.Results:The expression level of BMMC c-kit receptor mRNA and its receptor protein CD117 in CAA patients were higher than that in normal control group.And after treatment both of the expression levels descended to different degree.Moreover

the descent in Busuishengxue Granule group was superior to that in zaizhangshengxue Tablet group.Conclusions: There was abnormal expression of bone marrow SCF receptor c-kit/CD117 in CAA patients.Busuishengxue Granules by regulating the bone marrow c-kitmRNA and the expression of CD117 improved the hematopoietic function of bone marrow in CAA.

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Related Author

ZHANG Shou-lin1

QI De-jie2

SUN Wei-zheng1

ZHANG Yaoyin

ZHANG Chaochang

HE Jiaqi

WANG Wenru

DING Yubin

Related Institution

Xiyuan Hospital， China Academy of Chinese Medical Sciences

Graduate School of Beijing University of Chinese Medicine

Graduate School of China Academy of Chinese Medical Sciences

Dongfang Hospital， Beijing University of Chinese Medicine

Shenzhen Traditional Chinese Medicine（TCM） Hospital

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