Studies on Chemical Substances and Their Anticoagulant Activity of Qin-Lian-Si-Wu Decoction

LIU Chun-mei; TANG Yu-ping; GUO Jian-ming; WU De-kang; Ding An-wei~; DUAN Jin-ao

doi:10.13422/j.cnki.syfjx.2009.09.020

您当前的位置：

首页 >

文章列表页 >

Studies on Chemical Substances and Their Anticoagulant Activity of Qin-Lian-Si-Wu Decoction

更新时间：2024-09-23

- Studies on Chemical Substances and Their Anticoagulant Activity of Qin-Lian-Si-Wu Decoction
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 15, Issue 9, Pages: 54-58(2009)
- 作者机构：
  
  南京中医药大学江苏省方剂研究重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2009.09.020
  CLC： R285.5
- Published：2009
- 稿件说明：
移动端阅览
[1]刘春美,唐于平,郭建明,吴德康,丁安伟,段金廒.芩连四物汤化学物质基础及其抗凝血活性研究[J].中国实验方剂学杂志,2009,15(09):54-58.

LIU Chun-mei, TANG Yu-ping, GUO Jian-ming, et al. Studies on Chemical Substances and Their Anticoagulant Activity of Qin-Lian-Si-Wu Decoction[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(9): 54-58.
[1]刘春美,唐于平,郭建明,吴德康,丁安伟,段金廒.芩连四物汤化学物质基础及其抗凝血活性研究[J].中国实验方剂学杂志,2009,15(09):54-58. DOI： 10.13422/j.cnki.syfjx.2009.09.020.

LIU Chun-mei, TANG Yu-ping, GUO Jian-ming, et al. Studies on Chemical Substances and Their Anticoagulant Activity of Qin-Lian-Si-Wu Decoction[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(9): 54-58. DOI： 10.13422/j.cnki.syfjx.2009.09.020.

摘要

目的:研究芩连四物汤的化学物质基础及其抗凝血生物效应。方法:采用水煎醇沉

醇溶液减压回收乙醇

用适量水溶解后

上D101大孔树脂采用不同浓度的乙醇梯度洗脱

各不同洗脱部位采用硅胶柱层析的方法分离

所得单体通过UV

NMR及MS等方法确定其化学结构;将所得成分配成水溶液（100μmol.L-1）

采用体外法测定对血浆凝血酶时间（TT）的影响。结果:从芩连四物汤中分离并鉴定了5个黄酮类成分、1个生物碱、3个芳香酸、1个倍半萜类成分及2个其它类化合物。黄酮苷元、芳香酸、生物碱及倍半萜类化合物均能显著延长血浆凝血酶时间（TT）。结论:首次对芩连四物汤整方进行化学物质分离和结构分析研究

其中9个化合物

包括千层纸素A、汉黄芩素、6-甲氧基汉黄芩素、小檗碱、黄芩素、阿魏酸、香草酸、咖啡酸、地黄苦苷元显示了一定的体外抗凝血活性。

Abstract

Objective: To investigate the chemical constituents and anticoagulant activity of Qin-Lian-Si-Wu decoction.Methods: Qin-Lian-Si-Wu decoction was extracted by hot-water

and then concentrated by decompression

and added ethanol till the concentration. of ethanol reached 80%.The upper solution was concentrated at（40 ℃

） dissolved by distilled water

separated by gradient elution with different concentration ethanol from macroporius adsorptive resins D101

then obtained the different fraction.Its constituents were isolated by silica gel column chromatographic method

and their structures were identified through UV

NMR and MS spectrum.These compounds were prepared into aqueous solution

and then their effects on the blood plasma thrombin time in vitro in rabbits were determined.Result: Five flavonoids

one alkaloid

three aromatic acids

one sesquiterpene and two other components were isolated and identified from Qin-Lian-Si-Wu decoction.The results of thrombin time test showed that flavonoids

aromatic acids

alkaloid

sesquiterpenes could prolong the blood plasma thrombin time in vitro in rabbits remarkably.Conclusion: 12 compounds were isolated and identified from Qin-Lian-Si-Wu decoction for the first time.Nine compounds

including oroxylin A

wogonin

6-dimethoxy wogonin

berberine

baicalein

ferulic acid

vanillic acid

caffeic acid

rehmapicrogenin showed the anticoagulant activity at a certain dosage.

关键词

Keywords

references

Views

358

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

LIU Chun-mei

TANG Yu-ping

GUO Jian-ming

WU De-kang

DING An-wei

DUAN Jin-ao

Related Institution

No data

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰