Effect of "Weichangshu" on Gastro Smooth Muscle Cells in Rats of Diabetic Gastroparesis Model

ZHONG Yi~; ZHAO Zhi-ming; LU Ying-jie; LIAN Zhi-cheng

doi:10.13422/j.cnki.syfjx.2009.09.023

您当前的位置：

首页 >

文章列表页 >

Effect of "Weichangshu" on Gastro Smooth Muscle Cells in Rats of Diabetic Gastroparesis Model

更新时间：2024-09-23

- Effect of "Weichangshu" on Gastro Smooth Muscle Cells in Rats of Diabetic Gastroparesis Model
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 15, Issue 9, Pages: 71-77(2009)
- 作者机构：
  
  1. 广东省第二中医院消化科
  2. 广东省中医研究所
  3. 佛山科技学院
  4. 广州中医药大学脾胃研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2009.09.023
  CLC： R285.5
- Published：2009
- 稿件说明：
移动端阅览
[1]钟毅,赵自明,陆英杰,连至诚.胃肠舒对糖尿病胃轻瘫大鼠胃窦平滑肌细胞病变的影响[J].中国实验方剂学杂志,2009,15(09):71-77.

ZHONG Yi~, ZHAO Zhi-ming, LU Ying-jie, et al. Effect of "Weichangshu" on Gastro Smooth Muscle Cells in Rats of Diabetic Gastroparesis Model[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(9): 71-77.
[1]钟毅,赵自明,陆英杰,连至诚.胃肠舒对糖尿病胃轻瘫大鼠胃窦平滑肌细胞病变的影响[J].中国实验方剂学杂志,2009,15(09):71-77. DOI： 10.13422/j.cnki.syfjx.2009.09.023.

ZHONG Yi~, ZHAO Zhi-ming, LU Ying-jie, et al. Effect of "Weichangshu" on Gastro Smooth Muscle Cells in Rats of Diabetic Gastroparesis Model[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(9): 71-77. DOI： 10.13422/j.cnki.syfjx.2009.09.023.

摘要

目的:研究胃肠舒和血流干预药物对糖尿病胃轻瘫大鼠胃窦平滑肌细胞（SMC）病变的影响。方法:131只大鼠

随机分为正常组（10）、模型组（22）、止血敏组（33）、蚓激酶组（33）和胃肠舒组（33）

除正常组外大鼠1次性ip链脲佐菌素（60 mg.kg-1）造模

以7天后血糖≥16.7 mmol.L-1为纳入标准。3天后开始分别ig胃肠舒、止血敏i、p蚓激酶

模型组和正常组予蒸馏水ig。30周后检测血糖、碘[125I]-胰岛素（FINS）、碘[125I]-C肽（C-P）、胃液体排空率等。取胃窦组织

行HE染色、Mallory染色和免疫组化染色

光镜下观察胃窦组织SMC形态变化、SMC凋亡以及凋亡调控基因Bcl-2和Fax基因蛋白的表达。电镜下观察胃窦组织SMC超微结构改变。结果:30周后模型组大鼠胃窦SMC变性

SMC凋亡指数升高

与正常组比较差异显著（P<0.01）

SMC中Bcl-2基因蛋白表达减低、Fas基因蛋白过度表达

与正常组比较有显著性差异（P<0.01）。胃肠舒和蚓激酶治疗后胃窦平滑肌病变明显改善

而止血敏却使胃窦平滑肌病变加重。结论:糖尿病胃轻瘫存在着胃窦SMC病变

血流干预药物对其有影响

胃肠舒具有降糖、改善胃窦SMC病变、促进胃动力的作用。

Abstract

Objective:To study the effect of "Weichangshu" and medical interfering on blood flow and gastro smooth muscle cells in rats of diabetic gastroparesis model.Methods: One hundred thirty-one male SD rats（SPF class） were randomly divided into the control group（10 rats）

model group（22 rats）

ethamsylate group（33 rats）

lumbrukinase group（33 rats） and WCS group（33 rats）.Rats were intraperitoneally injected streptozotocin（STZ） solution(60 mg·kg

-1

).After seven days

we established blood sugar more than or equal to 16.7 mmol·L

-1

as experimental standard.Rats in the WCS group were treated by traditional Chinese medicine "WCS". So rats in the ethamsylate group treated by ethamsylate injection

rats in the lumbrukinase group treated by lumbrukinase enteric-coated capsules

rats in the control group and rats in the model group were treated by distilled water one time per day.After having been observed for 30 weeks

blood sugar

FINS

C-P and gastric fluid emptying rate were determined.Subsequently

samples of sinus ventriculi were made HE staining

Mallory staining and immunohistichemistry staining.Pathological lesion of gastric smooth muscle cells（SMC） was examined microscopically.Other sample of sinus ventriculi was made up electron microscopic specimen so as to know pathological lesion of ultrastructure of SMC through transmission electron microscope.Results: After 30 weeks

in the model group

endochylema of gastro smooth muscle cells were degenerated.The apoptotic index of SMC in rats of the model group was（24.33±2.34）% determined by TUNEL method

there was significantly difference compared with rats of the control group（P

0.01）.Protein expression of Bcl-2 in SMC was reduced in rats of the model group

but protein expression of Fas was overexpression

there was significantly difference compared with rats of the control group（P

0.01）.However

pathological lesion of gastro smooth muscle was obviously improved in rats of the WCS group and in rats of the lumbrukinase group.But in rats of the ethamsylate group pathological lesion of gastro smooth muscle was more serious.Conclusion:There were pathological Changes in gastric smooth muscle cells（SMC）in rats of diabetic gastroparesis model.Medicinal interfering in blood flow had the effect on gastric SMC pathology in rats of diabetic gastroparesis model."WCS" could decrease blood sugar and improve gastric SMC pathology and accelerate gastrointestinal motility.

关键词

Keywords

references

Views

257

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Hedysari Radix Polysaccharide Regulates Apoptosis of Smooth Muscle Cells in Gastric Antrum of Rat Model of Diabetic Gastroparesis via IGF-1/PI3K/Akt Signaling Pathway

Effect of Notoginseng Total Saponins on Apoptosis of Mammary Gland Cells in Rats with Mammary Gland Hyperplasia by Regulating PI3K/Akt/mTOR Pathway

Intervention Effect and Mechanism of Qingmei Compound on Acute Gouty Arthritis Based on NLRP3 Pathway

Effect of Huazhuo Jiedu Shugan Prescription on Learning， Memory， and PI3K/Akt/GSK-3β Signaling Pathway in Epileptic Rats

Related Author

ZHONG Yi

ZHAO Zhi-ming

LU Ying-jie

LIAN Zhi-cheng

MIAO Linlin

WAN Shengfang

ZHANG Lei

LI Rongke

Related Institution

School of Basic Medicine， Gansu University of Chinese Medicine

Gansu Key Laboratory of Traditional Chinese Medicine Formula Innovation and Transformation

Key Laboratory of Dunhuang Medicine， Ministry of Education， Gansu University of Chinese Medicine

Hebei Province Hospital of Traditional Chinese Medicine

Yantai New Era Health Industry Co.Ltd.

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰