LIU Yong-gang~1, ZHAO Bao-sheng~1, WANG Xiu-li~1, et al. Determination of Icariin in Xianling Gubao Dispersibie Tables by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(9): 6-7.
LIU Yong-gang~1, ZHAO Bao-sheng~1, WANG Xiu-li~1, et al. Determination of Icariin in Xianling Gubao Dispersibie Tables by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(9): 6-7. DOI: 10.13422/j.cnki.syfjx.2009.09.033.
Determination of Icariin in Xianling Gubao Dispersibie Tables by HPLC
摘要
目的:建立测定仙灵骨葆分散片中淫羊藿苷含量的高效液相色谱方法。方法:采用Agilent Extent C18(250 mm×4.6 mm
5μm)柱
以乙腈-0.1%乙酸溶液(28∶72)为流动相
流速:1.0 mL.min-1;在270 nm波长处检测。结果:淫羊藿苷在0.368
1
.84μg范围内呈线性关系
r=0.999 9;平均加样回收率为99.84%
RSD为1.59%(n=6)。结论:该方法简便快速
准确性和重复性好
可用于仙灵骨葆分散片中的淫羊藿苷的含量测定。
Abstract
Objective:To establish a method for HPLC determination of icariin in Xianling gubao Dispersibie Tables.Methods: The condition for HPLC was C
18
column(250 mm×4.6 mm
5 μm)
Agilent the detection wavelength was 270 nm.Results: The standard curve was liner in the range of 0.368~1.84 μg and the correlation coefficient was(0.999 9.)The coverage recovery was 99.84% with RSD 1.59%.Conclusion: The method shows good separating result
accuracy and reproducibility
can be used for the quality control of Xianling gubao Dispersibie Tables.