FANG Tie-zheng~, FU Su-ping. Determination the Content of Rutin in the Zhiyanxiao Keli by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(9): 14-15.
FANG Tie-zheng~, FU Su-ping. Determination the Content of Rutin in the Zhiyanxiao Keli by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(9): 14-15. DOI: 10.13422/j.cnki.syfjx.2009.09.036.
Determination the Content of Rutin in the Zhiyanxiao Keli by HPLC
摘要
目的:建立测定痔炎消颗粒中芦丁含量的方法。方法:采用高效液相色谱法。色谱柱为Merck Lichrospher C18柱(250 mm×4.6 mm
Objective:An HPLC method was established for the determination of Rutin.Methods:Using Merck Lichrospher C
18
(5 μm
250 mm×4.6 mm) with the mobile phase consisiting of methanol-1% glacial acetic acid(32∶68);The detective wavelength was 257 nm;The flow rate was 1 mL·min
-1
.Results:The standard curve was liner with the range of 0.27~7.11 μg(n=6
RSD=0.91%)
r=(0.999 5). The average recovery was 99.54%
RSD 0.91%.Conclusion:The method is simple and rapid which can be used for the quality control of Zhiyanxiao Keli.