Optimization of Extraction Conditions for the Total Flavonoids of Cacumen Platycladi by Orthogonal Design

HUANG Ying-hua~1; HUANG Yue-chun~1; WEI Gang~2; LIU Dong-hui~2; CHEN Mu-yuan~2

doi:10.13422/j.cnki.syfjx.2009.11.017

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Optimization of Extraction Conditions for the Total Flavonoids of Cacumen Platycladi by Orthogonal Design

更新时间：2024-09-23

- Optimization of Extraction Conditions for the Total Flavonoids of Cacumen Platycladi by Orthogonal Design
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 15, Issue 11, Pages: 34-37(2009)
- 作者机构：
  
  1. 广州中医药大学第一附属医院
  2. 广州中医药大学
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2009.11.017
  CLC： R284
- Published：2009
- 稿件说明：
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[1]黄樱华,黄月纯,魏刚,刘东辉,陈慕媛.正交试验法筛选侧柏叶总黄酮的提取工艺[J].中国实验方剂学杂志,2009,15(11):34-37.

HUANG Ying-hua~1, HUANG Yue-chun~1, WEI Gang~2, et al. Optimization of Extraction Conditions for the Total Flavonoids of Cacumen Platycladi by Orthogonal Design[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(11): 34-37.
[1]黄樱华,黄月纯,魏刚,刘东辉,陈慕媛.正交试验法筛选侧柏叶总黄酮的提取工艺[J].中国实验方剂学杂志,2009,15(11):34-37. DOI： 10.13422/j.cnki.syfjx.2009.11.017.

HUANG Ying-hua~1, HUANG Yue-chun~1, WEI Gang~2, et al. Optimization of Extraction Conditions for the Total Flavonoids of Cacumen Platycladi by Orthogonal Design[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(11): 34-37. DOI： 10.13422/j.cnki.syfjx.2009.11.017.

摘要

目的:研究侧柏叶总黄酮最佳提取工艺。方法:以总黄酮及槲皮苷的提取率为考察指标

采用L9（34）正交试验

考察提取溶媒、提取次数、提取时间、料液比因素对提取的影响

确定侧柏叶最佳提取工艺。结果:用70%乙醇为溶媒

以1∶8的料液比

回流3次

每次1.5 h为最佳提取条件。结论:该提取工艺方法简单、合理

是侧柏叶黄酮类化合物的最佳提取工艺。

Abstract

Objective:To study the optimum extraction process of the total flavonoids and quercitrin from cacumen platycladi.Methods: The extract conditions

such as concentration of ethanol

extraction times

extraction time and ratio of solvent to material were investigated by the orthonogal design with the content of tiliroside as the chemical reference.Results: The optimum extraction condition was established as follows: ethanol concentration 70%

extracting 1.5 hours for three times and the ratio of solvent to material 1∶8.Conclusion: The present investigation provided the optimum extraction process of the total flavonoids and quercitrin from the Platycladus orientalis Franco.

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Related Author

CHEN Mu-yuan2

LIU Dong-hui2

WEI Gang2

HUANG Yue-chun1

HUANG Ying-hua1

WEI Gang1

HUANG Yue-chun2

CHEN Mu-yuan1

Related Institution

Deptartment of New Drug R&D in Guangzhou University of Chinese Medicine

Dongguan Institution for Mathematics and Theoretics Engineering Research

Institute of Phytochemistry,Jilin Academy of Chinese Medicine Sciences

College of Pharmaceutical Sciences,Changchun University of Chinese Medicine

College of Pharmaceutical Sciences,Yanbian University,Yanji

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