Objective:To observe influence of White Prepared Lateral Root of Aconite on cardiac enzymes in normal and KidneyYang insufficiency rats and explore the difference of both.Method:Eighty Sprague-Dawley（SD）male rats were divided randomly into normal group and Kidney-Yang insufficiency model group

forty rats in each group.After producing Kidney-Yang insufficiency model by injecting hydrocortisoni natrii succinas in intramuscular

normal and model rats were respectively and randomly divided into little

low

medium

great dose group and blank group

eight rats in each group.Those rats were administered orally with different concentrations of white prepared lateral root of aconite every day for two weeks.After twenty-four hour the last oral administration

blood serum and cardiac muscle tissue homogenate were prepared and used for testing creatine kinase（CK）

lactate dehydrogenase（LDH）

cardiac muscle succinate dehydrogenase（SDH）

Na

+

-k

+

ATPase

adrenocorticotrophic hormone（ACTH）

hydrocortisone（COR）

cyclic adenosine monophosphate（cAMP）and cyclic guanosine monophosphate（cGMP）.Results:Hydrogenation cortisone caused cAMP and COR descend

but cGMP increase.With adding dose

all rats LDH squinted towards going up

normal rats’LDH going up was quicker than model rats’

which indicated mainly abnormal influence on surrounding cardiac muscle enzyme.However

all rats SDH and Na

+

-K

+

-ATPase had a trend of descending.Normal rats’ SDH descending was quicker than model rats’

which showed white prepared lateral root of aconite also impacted rats’cardiac muscle mitochondrion enzyme.Conclusion: White prepared lateral root of aconite had less cardiac toxicity on Kidney-Yang insufficiency rats.This study provided objective evidence for the theory of evaluating traditional Chinese herbal toxicity based on animal model with a specific TCM syndrome.