LIU Wei~, GUO Lei, FAN Ting, et al. Effect of Different Fertilizers on Content of Saikosaponin a、d in Radix Bupleuri[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(1): 18-21.
LIU Wei~, GUO Lei, FAN Ting, et al. Effect of Different Fertilizers on Content of Saikosaponin a、d in Radix Bupleuri[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(1): 18-21. DOI: 10.13422/j.cnki.syfjx.2010.01.010.
Effect of Different Fertilizers on Content of Saikosaponin a、d in Radix Bupleuri
Objective:Determine the content of Saikosaponin a and d by HPLC-ELSD.To analyze different methods of fertilization of the programme as optimum Fertilization one of the targets.Method:The HPLC method was used
chromatography condition was:Agilent TC-C
18
(4.6 mm×250 mm
5 μm)column
acetonitrile-water(50∶50) as mobile phase
detection is ELSD
drift temperature 93 ℃
air flow 2.5 L·min
-1
column temperature 20 ℃.Results:Establishing the fertilizer programme of Radix Bupleuri.Conclusion:The method is accuate
simple
and can provide the basis for optimization method of fertilization scheme by changes of Saikosaponin a and d.