Analysis of Chemical Composition of Dachuanxiong Fang by Ultra Performance Liquid Chromatography-Electrospray Ionization-Quadrupole Time of Flight/Mass Spectrometry

NI Shu-mao; QIAN Da-wei~; SHANG Er-xin; DUAN Jin-ao~; GUO Jian-ming; TANG Yu-ping

doi:10.13422/j.cnki.syfjx.2010.01.028

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Analysis of Chemical Composition of Dachuanxiong Fang by Ultra Performance Liquid Chromatography-Electrospray Ionization-Quadrupole Time of Flight/Mass Spectrometry

更新时间：2024-09-23

- Analysis of Chemical Composition of Dachuanxiong Fang by Ultra Performance Liquid Chromatography-Electrospray Ionization-Quadrupole Time of Flight/Mass Spectrometry
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 1, Pages: 39-45(2010)
- 作者机构：
  
  南京中医药大学江苏省方剂研究重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.01.028
  CLC： R284.1
- Published：2010
- 稿件说明：
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[1]倪书茂,钱大玮,尚尔鑫,段金廒,郭建明,唐于平.大川芎方化学成分的超高效液相色谱-电喷雾-四极杆飞行时间质谱分析[J].中国实验方剂学杂志,2010,16(01):39-45.

NI Shu-mao, QIAN Da-wei~, SHANG Er-xin, et al. Analysis of Chemical Composition of Dachuanxiong Fang by Ultra Performance Liquid Chromatography-Electrospray Ionization-Quadrupole Time of Flight/Mass Spectrometry[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(1): 39-45.
[1]倪书茂,钱大玮,尚尔鑫,段金廒,郭建明,唐于平.大川芎方化学成分的超高效液相色谱-电喷雾-四极杆飞行时间质谱分析[J].中国实验方剂学杂志,2010,16(01):39-45. DOI： 10.13422/j.cnki.syfjx.2010.01.028.

NI Shu-mao, QIAN Da-wei~, SHANG Er-xin, et al. Analysis of Chemical Composition of Dachuanxiong Fang by Ultra Performance Liquid Chromatography-Electrospray Ionization-Quadrupole Time of Flight/Mass Spectrometry[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(1): 39-45. DOI： 10.13422/j.cnki.syfjx.2010.01.028.

摘要

目的:采用液质联用技术（LC-MS）对中药大川芎方中的化学成分进行定性分析。方法:运用ACQUITY UPLCBEH C18（2.1 mm×100 mm

1.7μm）色谱柱

乙腈-水梯度洗脱;质谱使用ESI离子源

正离子与负离子模式下采集数据。结果:通过Q-TOFMS正、负离子质谱信息及元素组成分析并结合对照品与相关文献数据对照

共鉴定出17个化合物结构

主要结构类型为:酚类、有机酸类、苯酞类及含氮类化合物。同时

对大川芎方中主要化学成分的质谱裂解规律进行了分析。结论:经超高液相色谱分离

质谱测定相对分子质量及相关文献数据信息检索可以鉴别中药复方中的成分

为寻找大川芎方的物质溯源提供依据

为中药复方成分的鉴定提供一种快速准确的分析方法。

Abstract

Objective:To analyze and identify the chemical composition of Dachunxiong Fang by LC-MS.Method:The analysis was performed on an AQCUITY UPLC BEH C

（2.1 mm×100 mm

1.7 μm） column.The mobile phases were acetonitrile and water in gradient elution.ESI source was used and data were acquited in positive and negative mode.Result:The structures of 17 compounds of Dachunxiong Fang were identified by direct comparison with MS and MS

of Q-TOF

the elemental compositions analysis

the standard substance and the data of the literature.Furthermore

the types of the compounds were phenols

organic acids

phthalides and compounds containg nitrogen.Addition

the mass spectrum characterization of the major chemical composition of Dachunxiong Fang are analyzed.Conclusion:It is a rapid and accurate method that the composition of compound prescription of traditional Chinese medicine can be identified in terms of the separation of ultra high performance liquid chromatography

the accurate molecular weights measured by MS and other information

which can trace to the source of compounds in Dachuanxiong Fang.

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Related Author

TANG Yu-ping

GUO Jian-ming

SHANG Er-xin

QIAN Da-wei

NI Shu-mao

Lan SHEN

Shi-yu MA

Xiao LIN

Related Institution

School of Pharmacy, Shanghai University of Traditional Chinese Medicine(TCM)

Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine

Engineering Research Center of Modern Preparation Technology of TCM under Ministry of Education, Shanghai University of TCM

Lab.of Modern Preparation of Traditional Chinese Medicine,Jiangxi University of Traditional Chinese Medicine,Ministry of Education,Nanchang

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