MAO Yan, YANG Wei-jun, MA Yila·maimaitiyiming, et al. Determination of Adenosine in Weigan Effervescent Tablets by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 25-27.
MAO Yan, YANG Wei-jun, MA Yila·maimaitiyiming, et al. Determination of Adenosine in Weigan Effervescent Tablets by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 25-27. DOI: 10.13422/j.cnki.syfjx.2010.02.011.
Determination of Adenosine in Weigan Effervescent Tablets by RP-HPLC
摘要
目的:采用RP-HPLC法建立维感泡腾片中腺苷的含量测定方法。方法:以甲醇-水(8∶92)为流动相
ShimadzuC18(250 mm×4.6 mm
S-5μm)色谱柱
检测波长为260 nm
柱温为35℃
流速为1.0 mL.min-1。结果:腺苷在4.3
3
3.8μg范围内呈良好的线性关系(r=0.999 7)
腺苷的平均回收率为101.0%(n=9
RSD=1.4%)。结论:该方法快速简便易行
重复性良好
结果准确
可作为维感泡腾片中腺苷质量评价的方法。
Abstract
Objective:To develop a RP-HPLC method for the quantification of adenosine in Weigan Effervescent Tablets.Methods: The analysis was carried out on a Shimadzu C
18
column(250 mm×4.6 mm
S-5 μm)and the mobile phase was a mitture of methanol-water(8∶92)with a flow rate of 1.0 mL· min
-1
.The detection wavelength was 260 nm
and the column temperature was 35 ℃.Results: The linear response ranges of adenosine was 4.3~33.8 μg(r=(0.999 7))
and the average recovery was 101.0%(n=9
RSD=1.4%).Conclusion: The method is quick
simple and reproducible for the determination of adenosine in Weigan Effervescent Tablets.
Determination of Adenosine in Weigan Effervescent Tablets by RP-HPLC
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Related Author
MAO Yan
YANG Wei-jun
MA Yila·maimaitiyiming
HE Jin-hua
WANG Guo-hua
LI Hui
NIE Qi-xia
ZANG chen
Related Institution
Medical College of Shaoguan University
Shaoguan Institute for Drug Control
School of Pharmacy,Anhui Medical University
Department of Pharmacy,Third-Grade pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine,The First Affiliated Hospital of Anhui Medical University
Key Laboratory of Traditional Chinese Medicine Resource Science and Compound Prescription