Chemical Composition Analysis of Dangguibuxue Decoction by HPLC-DAD-MS

WANG Ping~

doi:10.13422/j.cnki.syfjx.2010.02.012

您当前的位置：

首页 >

文章列表页 >

Chemical Composition Analysis of Dangguibuxue Decoction by HPLC-DAD-MS

更新时间：2024-09-23

- Chemical Composition Analysis of Dangguibuxue Decoction by HPLC-DAD-MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 2, Pages: 28-31(2010)
- 作者机构：
  
  1. 大连医科大学
  2. 中南大学
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.02.012
  CLC： R284.1
- Published：2010
- 稿件说明：
移动端阅览
[1]王平,梁逸曾.基于HPLC-DAD-MS的当归补血汤化学成分分析[J].中国实验方剂学杂志,2010,16(02):28-31.

WANG Ping~. Chemical Composition Analysis of Dangguibuxue Decoction by HPLC-DAD-MS[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 28-31.
[1]王平,梁逸曾.基于HPLC-DAD-MS的当归补血汤化学成分分析[J].中国实验方剂学杂志,2010,16(02):28-31. DOI： 10.13422/j.cnki.syfjx.2010.02.012.

WANG Ping~. Chemical Composition Analysis of Dangguibuxue Decoction by HPLC-DAD-MS[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 28-31. DOI： 10.13422/j.cnki.syfjx.2010.02.012.

摘要

目的:分析中药复方当归补血汤的化学成分。方法:采用HPLC-DAD-MS方法分析。色谱柱为Thermo Hypersil-HypurityC18（150 mm×2.1 mm

5μm）分析柱

柱温40℃。流动相为H2O-CH3OH（梯度洗脱）

流速为0.2 mL.min-1。二极管阵列扫描范围:200

70 nm。质谱采用大气压化学电离（APCI+）离子源

扫描范围:100

50（m/z）。结果:通过与已有的文献报道的质谱、紫外光谱和保留行为比较可以初步定性10个化合物。结论:因为能够同时提供分子量、紫外光谱和保留行为

所以LC-DAD-MS是一个分析中药复方化学成分的有力工具。

Abstract

Objective:To study chemical components of Dangguibuxue decoction.Methods:The compounds were separated on a Hypersil-Hypurity C

（150 mm×2.1 mm

5 μm） analytical column.The column temperature was set at 40 ℃.Mobile phase consisted of methanol and water using a gradient elution.The flow rate was set at 0.2 mL·min

-1

.UV spectra were obtained by scanning from 200 nm to 370 nm.The APCI-MS spectra were obtained in the positive ion mode scanning from m/z 100 to 650.Results:The peaks were identified by comparing their UV spectra

their molecular weights and their retention behaviors with data reported in the literature.The ten components seem to be tentatively identified.Conclusions: Because of providing UV and molecular mass as well as retention time

LC-DAD-MS is a powerful tool for the chemical analysis of Traditional Chinese Medicines.

关键词

Keywords

references

Views

785

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Chemical Composition Analysis of Dangguibuxue Decoction by HPLC-DAD-MS

Optimizing the Extracting Process for Radix Astragali with Uniform Design

Studies on the Fingerprints of Radix Angelicae Sinensis

Study on Quality Standard of Jianshenhuayu Granules

The Effect of Fructus Lycii and Radix Astragali on Synthesis of Inhibin in Sertoli Cells of Rat Testis

Related Author

WANG Ping

LIANG Yi-zeng

QIAN Da-wei

ZHU Ling-ying

DUAN Jin-ao

JU Jian-ming

SHAO Wen-hao

Dong Zi-bo1

Related Institution

Chengdu University of Traditional Chinese Medicine,Ministry of Education Key Laboratory of Standardization of Chinese herbal medicines

Key Lab for Modern Preparation of Traditional Chinese Medicine,Ministry of Education of China,Jiangxi College of Traditional Chinese Medicine

Chengdu Medical College

Jiangsu Key Laboratory for TCM Formulae Research,Nanjing University of Chinese Medicine

Bethune International Peace Hospital

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰