Dertermination of Palmatine and Berberine in Shulitong Granule by HPLC

CAI Qing-yu~1; ZHANG Yi~1; HAO Te~1; TONG Hui-juan~2; KANG Chen~2; LI Man-ling~

doi:10.13422/j.cnki.syfjx.2010.02.018

您当前的位置：

首页 >

文章列表页 >

Dertermination of Palmatine and Berberine in Shulitong Granule by HPLC

更新时间：2024-09-23

- Dertermination of Palmatine and Berberine in Shulitong Granule by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 2, Pages: 42-44(2010)
- 作者机构：
  
  1. 海军总医院药剂科
  2. 中国中医科学院中药研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.02.018
  CLC： R286.0
- Published：2010
- 稿件说明：
移动端阅览
[1]蔡清宇,张沂,郝特,仝会娟,康琛,李曼玲.HPLC法测定舒利通颗粒中巴马丁和小檗碱的含量[J].中国实验方剂学杂志,2010,16(02):42-44.

CAI Qing-yu~1, ZHANG Yi~1, HAO Te~1, et al. Dertermination of Palmatine and Berberine in Shulitong Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 42-44.
[1]蔡清宇,张沂,郝特,仝会娟,康琛,李曼玲.HPLC法测定舒利通颗粒中巴马丁和小檗碱的含量[J].中国实验方剂学杂志,2010,16(02):42-44. DOI： 10.13422/j.cnki.syfjx.2010.02.018.

CAI Qing-yu~1, ZHANG Yi~1, HAO Te~1, et al. Dertermination of Palmatine and Berberine in Shulitong Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 42-44. DOI： 10.13422/j.cnki.syfjx.2010.02.018.

摘要

目的:建立以高效液相色谱法测定舒利通颗粒中巴马丁和小檗碱含量的方法。方法:色谱柱:Kromasil-C18（250mm×4.6 mm

5μm）;流动相:乙腈-0.1 mol.L-1磷酸二氢钾溶液（28∶72）;流速:1 mL.min-1;检测波长:345 nm;柱温:室温。结果:盐酸巴马丁在0.016

.080μg范围内（r=0.999 8）

盐酸小檗碱在0.027

.135μg范围内（r=0.999 9）呈良好的线性关系。盐酸巴马丁平均回收率为99.74%

RSD=2.67%（n=5）;盐酸小檗碱平均回收率为103.53%

RSD=1.48%（n=5）。结论:此方法简捷、准确性、重复性好、专属性强

可供用于舒利通颗粒的质量标准研究。

Abstract

Objective:To develop a HPLC method for the determination of palmatine and berberine in Shulitong Granule.Methods:A Kromasil-C

（250 mm×4.6 mm

5 μm）column was used for the HPLC determination.The mobile phase consisted of acetonitrile-0.1 mol/L potassium dihydrogen phosphate（28∶72）

and the flow rate was 1 mL/min.The detection wavelength was 345 nm.Results:A good linear relationship was constructed in the range of 0.016～0.080 μg（r=（0.999 8））

for Palmatine hydrochloride and in the range of 0.027～0.135 μg（r=（0.999 9）） for berberine hydrochloride. The average recovery of palmatine hydrochloride was 99.74%（RSD=2.67%

n=5）

and that of berberine hydrochloride was 103.53%

RSD=1.48%（n=5）.Conclusion:The developed method is simple

reproducible

specific and suitable for the quality control of Shulitong Granule.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Dertermination of Palmatine and Berberine in Shulitong Granule by HPLC

Preparation and Quality Control of Standard Extract from Coptidis Rhizoma

Quality Standard of Xiao’er Xieli Tablet

Optimization of Extraction Technology for Sanhuang Pill by Orthogonal Test

Determination of Berberine Hydrochloride,Baicalin and Wogonin in Yankening Pian by HPLC

Related Author

CAI Qing-yu

ZHANG Yi

HAO Te

TONG Hui-juan

KANG Chen

LI Man-ling

ZHANG Hong-mei

WANG Chang-hong

Related Institution

Pharmacy Faculty， Hubei University of Chinese Medicine

Hubei Engineering Technology Research Center of Chinese Material Medical Processing

Tongji Hospital， Tongji Medical College of Huazhong University of Science and Technology

College of Pharmacy, School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine

Tibetan Medical College, Qinghai University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰