ESR study and Protection of Water Extract of Carthamus tinctorius L. on Injury of Cardiomyocytes induced by Hydrogen Peroxide

YE Jin-xia~

doi:10.13422/j.cnki.syfjx.2010.02.032

您当前的位置：

首页 >

文章列表页 >

ESR study and Protection of Water Extract of Carthamus tinctorius L. on Injury of Cardiomyocytes induced by Hydrogen Peroxide

更新时间：2024-09-23

- ESR study and Protection of Water Extract of Carthamus tinctorius L. on Injury of Cardiomyocytes induced by Hydrogen Peroxide
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 2, Pages: 56-59(2010)
- 作者机构：
  
  1. 中国中医科学院中药研究所
  2. 福建中医学院中西医结合研究院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.02.032
  CLC： R285.5
- Published：2010
- 稿件说明：
移动端阅览
[1]叶锦霞,王岚,杨滨,梁日欣.红花水提物对乳鼠心肌细胞H_2O_2所致损伤的保护作用及ESR谱研究[J].中国实验方剂学杂志,2010,16(02):56-59.

YE Jin-xia~. ESR study and Protection of Water Extract of Carthamus tinctorius L. on Injury of Cardiomyocytes induced by Hydrogen Peroxide[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 56-59.
[1]叶锦霞,王岚,杨滨,梁日欣.红花水提物对乳鼠心肌细胞H_2O_2所致损伤的保护作用及ESR谱研究[J].中国实验方剂学杂志,2010,16(02):56-59. DOI： 10.13422/j.cnki.syfjx.2010.02.032.

YE Jin-xia~. ESR study and Protection of Water Extract of Carthamus tinctorius L. on Injury of Cardiomyocytes induced by Hydrogen Peroxide[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 56-59. DOI： 10.13422/j.cnki.syfjx.2010.02.032.

摘要

目的:观察红花水提物对体外培养大鼠乳鼠心肌细胞H2O2损伤的保护作用及电子自旋共振（ESR）波谱的影响。方法:建立心肌细胞H2O2损伤模型

实验分5组

空白对照组

模型对照组:H2O2（1 mmol.L-1）孵育1 h;红花水提物500

100

20 mg.L-1剂量组:分别给予不同浓度的红花水提物

预处理24 h

然后换为H2O2（1 mmol.L-1）孵育1 h。实验结束后分别收集细胞上清液测定乳酸脱氢酶（LDH）、丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、一氧化氮（NO）、一氧化氮合酶（NOS）和黄嘌呤氧化酶（XOD）活性

用自旋捕集技术结合电子自旋共振波谱技术（Electron Spin Res-onance

简称ESR）检测各组细胞悬液中氧自由基信号的强弱。结果:红花水提物各剂量组能够明显提高H2O2损伤后的心肌细胞的搏动频率

使细胞存活率提高

减少心肌细胞LDH的释放

降低培养液中MDA和XOD的含量

同时提高SOD

NO和GSH-Px的活性

使细胞悬液中的ESR氧自由基信号明显减弱。结论:红花水提物对体外培养乳鼠心肌细胞具有保护作用

其机制与其减少活性氧产生、增强氧自由基的清除、增强内源性抗氧化酶的活性有关。

Abstract

Objective:To investigate the antioxidant effects of water extract of Carthamus tinctorius L.on injury of cardiomyocytes induced by hydrogen peroxide（H

） and to detect reactive oxygen species（ROS） for exploring the mechanisms.Methods:In cultured neonatal rat cardiomyocytes

the antioxidant effect of water extract of Carthamus tinctorius L.was investigated after H

(1 mmol·L

-1

) induced damage.The spontaneous beating

the survival rate and release of LDH

MDA

SOD

NOS

GSH-Px and XOD enzyme activity in supernatant were investigated

and cell suspension was collected for detecting reactive oxygen species（ROS） by electron spin resonance（ESR）.Results: Preconditioning by water extract of Carthamus tinctorius L.increased the spontaneous beating and the survival rate

and decreased the release of LDH

MDA and SOD with the rise of SOD

NOS and GSH-Px enzyme activity

while in the cell suspension ROS signal decreased significantly.Conclusion: Water extract of Carthamus tinctorius L.has antioxidative effect.The mechanisms are likely related with scavenging of free radicals

enhancing its clearance

enhancing endogenous antioxidant activity.

关键词

Keywords

references

Views

281

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

ESR study and Protection of Water Extract of L. on Injury of Cardiomyocytes induced by Hydrogen Peroxide

Effect of Didang Xianxiong Decoction on Myocardium Ultrastructure and Expression of CTGF and Its Receptor LRP in Diabetic Rats

Mechanism of Trichosanthis Pericarpium Aqueous Extract in Protecting H9c2 Cardiomyocytes from Hypoxia/Reoxygenation Injury via PI3K/Akt/NO Signaling Pathway

Protective Effects of Total Saponin from on HO Induced Oxidative Stress in Neonatal Rat Cardiomyocytes

Related Author

YE Jin-xia

WANG Lan

YANG Bin

LIANG Ri|xin

CAI Zhengyin

CHU Jun

CHU Quangen

XUAN Yun

Related Institution

Anhui University of Chinese Medicine

Key Laboratory of Xin'an Medicine， Ministry of Education， Anhui University of Chinese Medicine

School of Biomedical and Chemical Engineering, Liaoning Institute of Science and Technology

School of Pharmacy, Liaoning University of Traditional Chinese Medicine

Department of Pharmacology,Institute of Traditional Chinese Medicine of Dalian

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰