The Experimental Study on the mRNA Expression of CYP11B2 in Rat with Kidney Deficiency and Diuresis Induced by Adenine

LI Shu-wen~

doi:10.13422/j.cnki.syfjx.2010.02.034

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The Experimental Study on the mRNA Expression of CYP11B2 in Rat with Kidney Deficiency and Diuresis Induced by Adenine

更新时间：2024-09-23

- The Experimental Study on the mRNA Expression of CYP11B2 in Rat with Kidney Deficiency and Diuresis Induced by Adenine
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 2, Pages: 64-67(2010)
- 作者机构：
  
  1. 江西中医药高等专科学校
  2. 广州中医药大学中药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.02.034
  CLC： R285.5
- Published：2010
- 稿件说明：
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[1]李淑雯,吴清和,黄萍,操红缨.腺嘌呤肾虚多尿大鼠肾脏CYP11B2 mRNA表达变化的研究[J].中国实验方剂学杂志,2010,16(02):64-67.

LI Shu-wen~. The Experimental Study on the mRNA Expression of CYP11B2 in Rat with Kidney Deficiency and Diuresis Induced by Adenine[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 64-67.
[1]李淑雯,吴清和,黄萍,操红缨.腺嘌呤肾虚多尿大鼠肾脏CYP11B2 mRNA表达变化的研究[J].中国实验方剂学杂志,2010,16(02):64-67. DOI： 10.13422/j.cnki.syfjx.2010.02.034.

LI Shu-wen~. The Experimental Study on the mRNA Expression of CYP11B2 in Rat with Kidney Deficiency and Diuresis Induced by Adenine[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 64-67. DOI： 10.13422/j.cnki.syfjx.2010.02.034.

摘要

目的:通过研究腺嘌呤对醛固酮合成酶途径的影响

探讨其导致"肾虚多尿"的机制所在。方法:利用酶联免疫分析法分别检测正常组、腺嘌呤组动物血中皮质酮（Cort）、醛固酮（ALD）的含量;并采用RT-PCR的方法检测各组动物肾脏CYP11B2 mRNA的表达。结果:与正常组比较

腺嘌呤可明显减少大鼠血中Cort

ALD含量

并下调模型大鼠肾脏CYP11B2mRNA的表达。结论:腺嘌呤肾虚多尿大鼠血中Cort

ALD含量减少

肾脏CYP11B2 mRNA的表达下调

影响醛固酮合成酶途径

从而减少ALD的合成

导致水液代谢异常引发多尿

本实验研究结果从醛固酮合成酶的角度阐明了腺嘌呤导致"肾虚多尿"的作用机制。

Abstract

Objective:To explore the mechanism for kidney deficiency and diuresis by studying the action of adenine to ALD synthase.Methods:The contents of Cort

ALD in blood were determined and the mRNA expression of CYP11B2 was evaluated with ELISA and RT-PCR in model group and control group.Results:Compared with control group

adenine could remarkedly decrease the contents of Cort

ALD in rat blood;and downregulate the mRNA expression of CYP11B2.Conclusion:Adenine can decrease the contents of Cort

ALD in blood and downregulate the mRNA expression of CYP11B2 in rats.It can decrease the synthesis of ALD by adjusting the ALD synthase.The results may expound the mechanism of kidney deficiency and diuresis induced by adenine from the angle of ALD synthase approach.

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Related Author

CAO Hong-ying

HUANG Ping

WU Qing-he

LI Shu-wen

GUO Dengzhou

LI Wanzhe

JIN Peipei

ZHANG Yuanyuan

Related Institution

Graduate School， Hebei University of Chinese Medicine

The First Affiliated Hospital of Hebei University of Chinese Medicine，Hebei Provincial Hospital of Traditional Chinese Medicine

Affiliated Hospital of Hebei University of Chinese Medicine/Hebei Provincial Hospital of Traditional Chinese Medicine

School of Pharmacy,Chinese Materia Medica Processing Engineering Center of Liaoning Province,Liaoning University of Traditional Chinese Medicine

Shanxi University of Traditional Chinese Medicine

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