The extraction and determination the contents of astragaloside

WANG Pei-pei1; XU Du-juan2; XIA Quan2

doi:10.13422/j.cnki.syfjx.2010.04.019

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The extraction and determination the contents of astragaloside

更新时间：2024-09-23

- The extraction and determination the contents of astragaloside
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 4, Pages: 27-30(2010)
- 作者机构：
  
  1. 安徽医科大学药学院
  2. 安徽医科大学第一附属医院药剂科国家中医药管理局中药化学三级实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.04.019
  CLC： R284.1
- Published：2010
- 稿件说明：
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[1]王培培,许杜娟,夏泉.黄芪皂苷的提取及含量测定[J].中国实验方剂学杂志,2010,16(04):27-30.

WANG Pei-pei1, XU Du-juan2, XIA Quan2. The extraction and determination the contents of astragaloside[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 27-30.
[1]王培培,许杜娟,夏泉.黄芪皂苷的提取及含量测定[J].中国实验方剂学杂志,2010,16(04):27-30. DOI： 10.13422/j.cnki.syfjx.2010.04.019.

WANG Pei-pei1, XU Du-juan2, XIA Quan2. The extraction and determination the contents of astragaloside[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 27-30. DOI： 10.13422/j.cnki.syfjx.2010.04.019.

摘要

目的:研究黄芪药材中黄芪皂苷的提取工艺及含量测定。方法:采用70%乙醇回流提取

水饱和正丁醇萃取

D101大孔吸附树脂富集纯化黄芪总皂苷

硅胶柱层析分离不同的皂苷组分;采用薄层色谱法（TLC）、高效液相-示差检测器法（HPLC-RID）进行皂苷类成分的鉴定和含量测定。结果:从黄芪总皂苷中分离出六种黄芪皂苷化合物（AS-Ⅰ～AS-Ⅵ）

其中AS-Ⅰ部分经鉴定主要含皂苷Ⅱ

质量分数为29.4%

AS-Ⅲ部分经鉴定主要含皂苷Ⅳ

质量分数为22.82%。其余4个部分有待于进一步鉴定。结论:此法能较好的分离和纯化黄芪皂苷

TLC和HPLC-RID法操作简便

可作为黄芪皂苷的鉴定和含量测定方法。

Abstract

Objective:Study on extracting technology and assay of astragaloside in Astragalus.Method: 70% ethanol circumfluence extraction was used.Total astragaloside was enriched and purified by extraction refine through water saturated of n-butanol and D101 mcroporous adsorption resin.Different components of astragaloside were isolated by silica gel laminar analysis.Identification by using TLC method and determination the contents by u-sing HPLC-RID Method.Result: Six compounds were isolated from total astragaloside（AS-Ⅰ ～ AS-Ⅵ）

and 29.4% astragaloside Ⅱ was identified in As-Ⅰ part.AS-Ⅲ which content is 22.82% is mainly contained Astraga-losideⅣ by identification.The rest four compounds are required to be identified.Conclusion:The method can be used for astragaloside

HPLC-RID method can be used to determine the content of astragaloside.

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