WEI Hui-zhen1, LI Xin-nan2, GONG Jian-ping1, et al. Evaluation of measurement uncertainty for the determination of total flavonoids in Luoyin soft capsules by UV spectrometery method[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 39-43.
WEI Hui-zhen1, LI Xin-nan2, GONG Jian-ping1, et al. Evaluation of measurement uncertainty for the determination of total flavonoids in Luoyin soft capsules by UV spectrometery method[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 39-43. DOI: 10.13422/j.cnki.syfjx.2010.04.024.
Evaluation of measurement uncertainty for the determination of total flavonoids in Luoyin soft capsules by UV spectrometery method
Objective:To evaluate the uncertainty of measurement for determination of total flavonoids in Luoyin soft capsules by UV spectrometery.Method:Through establishing UV spectrometery method determination mode
the factors affecting the uncetainty were deduced
including standard solution preparation
sample pretreat-ment
color reaction
calibration curve and recovery tests.Each component of uncertainty was evaluated
from which the combined uncertainty and finally the extended uncertainty were obtaine in 95% confidence intervals.Result: The determined content of total flavonoids in Luoyin soft capsules of this test is(31.13 ± 1.61) mg.g-1.Conclu-sion:The established mathematic model is applicable to the uncertainty analysis of UV spectrometery method in the determination of traditional Chinese medicine.