SONG Xiao-dong, ZHANG Jin-jin, LIU Wen-bo, et al. Effect of Chinese Medicine Weichangshu on Mitochondrial in Diabetic Mellitus Rats Stomach Smooth Mucle Cells[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 118-122.
SONG Xiao-dong, ZHANG Jin-jin, LIU Wen-bo, et al. Effect of Chinese Medicine Weichangshu on Mitochondrial in Diabetic Mellitus Rats Stomach Smooth Mucle Cells[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 118-122. DOI: 10.13422/j.cnki.syfjx.2010.05.015.
Effect of Chinese Medicine Weichangshu on Mitochondrial in Diabetic Mellitus Rats Stomach Smooth Mucle Cells
Western Blot检测线粒体蛋白的变化。结果:胃肠舒能改善DM大鼠胃窦平滑肌细胞线粒体的形态、结构
增强呼吸链功能
线粒体跨膜电位和ATP含量增高
细胞色素C氧化酶(cytochrome Coxidase
COX)活性增强
活化物氧自由基(ROS)释放减少
凋亡蛋白家族Bcl-2表达上调
Bax表达下调。结论:胃肠舒促胃肠运动可能与改善平滑肌的线粒体动能系统有关。
Abstract
Objective:To study the mechanism of the chinese medicine Weichangshu on mitochondrial kinetic energy in diabetic mellitus rats stomach by measuring the changes of morphology
structure
function and protein of mitochondria. Method: Standard Sprague Dawley rats with a mean weight of 150-170g were randomly divided into 4 groups
including a control group
DM model group
DM + Weichangshu Ⅰ group
DM + Weichangshu Ⅱ group. The mitochondrial morphology and structure were detected using transmission electron microscopy. The changes of mitochondrial transmembrane potential
respiratory chain and mitochondrial protein were detected using flow cytometry
fluorescence labeling and Western Blot respectively. Result: Weichangshu could ameliorate the mitochondrial morphology
structure and respiratory chain in diabetic mellitus rats stomach. Mitochondrial transmembrane potential
ATP level and activity of cytochrome C oxidase were increased. The release of ROS was decreased. The expression of Bcl-2 up-regulated and bax down-regulated. Conclusion:Weichangshu could promoted the gastrointestinal motility
which might be related with the regulation of mitochondrial kinetic energy.
Effect of Chinese Medicine Weichangshu on Mitochondrial in DiabeticMellitus Rats Stomach Smooth Mucle Cells
Effect of Weichangshu on Mitochondrial of Stomach Smooth Muscle Cells in Rats with Diabetic Mellitus
Effect of Weichangshu on Mitochondrial of Stomach Smooth Muscle Cells in Rats with Diabetic Mellitus
Experimental Research Progress of Replenishing Qi and Activating Blood Circulation Traditional Chinese Medicine on Mitochondrial Quality Control in Treatment of Chronic Heart Failure
Effect of Rhei Radix et Rhizoma on Gastrointestinal Function of Normal Rats Before and After Simmering
Related Author
WANG Xiu-wen
LIU Wen-bo
ZHANG Jin-jin
SONG Xiao-dong
WANG Xiu-wen
LIU Wen-bo
ZHANG Jin-jin
SONG Xiao-dong
Related Institution
Experiment Center of Binzhou Medical College
Graduate School,Key Laboratory of Xin'an Medicine,Ministry of Education, Anhui University of Chinese Medicine
The First Affiliated Hospital of Anhui University of Chinese Medicine
The Second Affiliated Hospital of Anhui University of Chinese Medicine
School of Pharmacy, Hubei University of Chinese Medicine