GONG Ming-yu1, ZHANG Li2, DU Chao1, et al. Influence of Breviscapine on Ultrastructure and Apoptosis of Myocardial Cells after Myocardial Ischemia and Eperfusion in Rat[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 147-149.
GONG Ming-yu1, ZHANG Li2, DU Chao1, et al. Influence of Breviscapine on Ultrastructure and Apoptosis of Myocardial Cells after Myocardial Ischemia and Eperfusion in Rat[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 147-149. DOI: 10.13422/j.cnki.syfjx.2010.05.025.
Influence of Breviscapine on Ultrastructure and Apoptosis of Myocardial Cells after Myocardial Ischemia and Eperfusion in Rat
Objective: To explore the effect and mechanism of breviscapine against myocardial ischemia/ reperfusion injury in rat. Method: 40 SD rats were randomly divided into sham operation group
ischemia / reperfusion group and breviscapine group. Rats in breviscapine group were injected with breviscapine 25
50 mg· kg1· d -1 respectively prior to ischemia /reperfusion. The other two groups were given normal saline instead of breviscapine equally in volume at a week before the operation. The myocardial ischemia /reperfusion model was established by ligating the left anterior descending branch of coronary artery. Apoptosis of myocytes was detected after operation in all groups by flow cytometry with Annexin Ⅴ and propidium iodiode ( PI ) staining. The ultrastructural alterations were observed under the transmission electron microscopy. Result: breviscapine could decrease the occurrence rate of myocardial apoptosis during ischemia /reperfusion injury obviously( P < 0. 05
P < 0. 01). The degree of injury on ultrastructures of myocardial cell in breviscapine group is rather alleviated compared with that in the ischemia /reperfusion group. Conclusion: breviscapine may protect myocardial cells during ischemia /reperfusion injury and the protective mechanism may be achieved by inhibiting myocardial apoptosis.