Determination of Oleanic Acid and Ursonic Acid in Crude Hawthorn Berry by HPLC

CHEN Bao-long1; FENG Kun1; ZHENG Zhao-hua2; CHEN Yu-ying2

doi:10.13422/j.cnki.syfjx.2010.05.034

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Determination of Oleanic Acid and Ursonic Acid in Crude Hawthorn Berry by HPLC

更新时间：2024-09-23

- Determination of Oleanic Acid and Ursonic Acid in Crude Hawthorn Berry by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 5, Pages: 73-75(2010)
- 作者机构：
  
  1. 河南省正骨研究院
  2. 中国药科大学分析化学教研室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.05.034
  CLC： R284.1
- Published：2010
- 稿件说明：
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[1]陈宝龙,冯坤,郑朝华,陈玉英.山楂中齐墩果酸和熊果酸的测定[J].中国实验方剂学杂志,2010,16(05):73-75.

CHEN Bao-long1, FENG Kun1, ZHENG Zhao-hua2, et al. Determination of Oleanic Acid and Ursonic Acid in Crude Hawthorn Berry by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 73-75.
[1]陈宝龙,冯坤,郑朝华,陈玉英.山楂中齐墩果酸和熊果酸的测定[J].中国实验方剂学杂志,2010,16(05):73-75. DOI： 10.13422/j.cnki.syfjx.2010.05.034.

CHEN Bao-long1, FENG Kun1, ZHENG Zhao-hua2, et al. Determination of Oleanic Acid and Ursonic Acid in Crude Hawthorn Berry by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 73-75. DOI： 10.13422/j.cnki.syfjx.2010.05.034.

摘要

目的:建立测定山楂果中齐墩果酸和熊果酸含量的高效液相色谱法。方法:采用C18色谱柱

甲醇-水-磷酸-三乙胺（87∶13∶0.2∶0.04）为流动相

流速0.9mL·min-1

35℃

检测波长210nm。结果:齐墩果酸和熊果酸的分离度>1.4;方法学考察符合液相色谱法的要求;山楂药材中齐墩果酸含量>0.5mg·g-1;熊果酸含量>1.9mg·g-1。结论:该法简便、灵敏、分离度高、专属性好

适用于山楂中齐墩果酸和熊果酸的含量测定。

Abstract

Objective:To establish an HPLC method for determination the content of Oleanic acid and Ursonic acid in crude hawthorn berry. Method: The HPLC analysis was carried out through C18 column with detection wavelength set at 210 nm and the temparature set at 35 ℃ . The mobile phase was composed of MeOHH2O-H3PO4-TEA （87∶ 13∶ 0. 2∶ 0. 04） at a flow rate of 0. 9 mL·min -1. Result:The separation of Oleanic acid and Ursonic acid was 1. 4; the methodologic validation conformed to requirement of liquid chromato-graphy. The content of Oleanic acid and Ursonic acid in crude hawthorn berry was more than 0. 5 mg·g -1 and 1. 9 mg·g -1

respectively. Conclusion:The HPLC method can be used in the routine analysis of Oleanic acid and Ursonic acid in crude hawthorn berry.

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Related Institution

Department of Pharmacy,First Affiliated Hospital of PLA General Hospital

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