Antioxidant Activity and Inhibition on Acetylcholinesterase by Huanglianjiedu Decoction

SONG Jian-fang; WANG Hong-jie; SI Nan; BIAN Bao-lin

doi:10.13422/j.cnki.syfjx.2010.05.038

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Antioxidant Activity and Inhibition on Acetylcholinesterase by Huanglianjiedu Decoction

更新时间：2024-09-23

- Antioxidant Activity and Inhibition on Acetylcholinesterase by Huanglianjiedu Decoction
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 5, Pages: 61-64(2010)
- 作者机构：
  
  中国中医科学院中药研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.05.038
  CLC： R285.5
- Published：2010
- 稿件说明：
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[1]宋建芳,王宏洁,司南,边宝林.黄连解毒汤的抗氧化作用及抑制乙酰胆碱酯酶活性的研究[J].中国实验方剂学杂志,2010,16(05):61-64.

SONG Jian-fang, WANG Hong-jie, SI Nan, et al. Antioxidant Activity and Inhibition on Acetylcholinesterase by Huanglianjiedu Decoction[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 61-64.
[1]宋建芳,王宏洁,司南,边宝林.黄连解毒汤的抗氧化作用及抑制乙酰胆碱酯酶活性的研究[J].中国实验方剂学杂志,2010,16(05):61-64. DOI： 10.13422/j.cnki.syfjx.2010.05.038.

SONG Jian-fang, WANG Hong-jie, SI Nan, et al. Antioxidant Activity and Inhibition on Acetylcholinesterase by Huanglianjiedu Decoction[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 61-64. DOI： 10.13422/j.cnki.syfjx.2010.05.038.

摘要

目的:筛选黄连解毒汤中与治疗老年性疾病有关的有效成分。方法:运用紫外分光光度法

建立体外抗氧化模型和抑制乙酰胆碱酯酶（AchE）活性实验模型

分析比较了黄连解毒汤全方、单味药及其主要成分的抗氧化作用和对乙酰胆碱酯酶的抑制作用。结果:复方黄连解毒汤的抗氧化活性比黄连、黄柏和栀子单味药强;黄芩及其主要成分黄芩苷（IC50=12.06μg·mL-1）具有较强的抗氧化活性;与对照品毒扁豆碱和加兰他敏比较

小檗碱（IC50=9.52μg·mL-1）对乙酰胆碱酯酶的抑制活性较高

其次是黄连（IC50=18.89μg·mL-1）水提物

而黄芩和栀子抑制活性不明显。结论:黄连解毒汤具有抗氧化和抑制乙酰胆碱酯酶活性作用

其中具有多羟基的黄酮类是抗氧化活性的主要成分;而生物碱类则是抑制乙酰胆碱酯酶的主要成分。

Abstract

Objective: To investigate and screen the active compounds of Huanglianjiedu decoction（HLJD） for senile dementia. Method: Inhibition on acetylcholinesterase and antioxidant activity by HLJD was studied with ultraviolet spectrometry. The single herbs and the major compounds of HLJD were also determined and compared by a rapid and effective acetylcholinesterase inhibition assay and antioxidant assay. Result: The formula HLJD had a better antioxidant activity than single herbs except for Radix Scutellaria which is rich in polyphenolic compounds. The most active conpounds was baicalin （ IC50 = 12. 06 μg·mL -1 ）. The results also indicate that the most active herbs and compounds for AChE inhibition were Coptidis Rhizoma （IC50 = 18. 89 μg·mL -1） and berberine （ IC50 = 9. 52 μg·mL -1）; Radix Scutellariae

Fructus Gardenia as well as the major compounds of them showed no or lower inhibitory activity. Conclusion: It can be concluded that alkaloid compounds in HLJD contribute to the inhibitory activity for acetylcholinesterase and flavanoids compounds contribute to the antioxidant activity.

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Related Author

SONG Jian-fang

WANG Hong-jie

SI Nan

BIAN Bao-lin

HAO Yu

FANG Su-ping

QIU Quan-ying

WANG De-sheng

Related Institution

School of Chinese Pharmacy,Beijing University of Chinese Medicine

Department of Neurology,the First Hospital,Harbin Medical University

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