LI Xi-feng, HAO Zhe, QIU Tian-bao, et al. Determination of Oleanolic Acid in dandelion by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 52-54.
LI Xi-feng, HAO Zhe, QIU Tian-bao, et al. Determination of Oleanolic Acid in dandelion by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 52-54. DOI: 10.13422/j.cnki.syfjx.2010.05.042.
Determination of Oleanolic Acid in dandelion by RP-HPLC
Objective:To establish the determination method for oleanolic acid in dandelion by HPLC. Method: HPLC was carried out on Hypersil ODS2 C18 (4. 6 mm × 250 mm
5 μm) at 30 ℃ using methanol-0. 5% H3PO4 (pH = 3. 5)(90∶ 10) as mobile phase with a flow rate of 1. 0 mL·min -1 and detection wavelength was at 210 nm. Result:The oleanolic acid was linear within the range of 0. 872 ~ 7. 848 μg. The average recovery was 98. 25% and RSD was 1. 54% . Conclusion:This method is useful for quality control of dandelion.
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Related Author
LI Xi-feng
HAO Zhe
QIU Tian-bao
YU Yun-hui
DU Yun-feng
LI Xi-feng
HU Ya-nan
SHI Yang
Related Institution
School of Pharmaceutical Sciences, Changchun University of Chinese Medicine
Jilin Ginseng Academy,Changchun University of Chinese Medicine
Chengdu University of Traditional Chinese Medicine