Analysis of Anti-hepatic Fibrosis Effects of WuLing Capsules Compatibility and Ingredients by Herbal with Drawal Analysis

HU Yong-wu; WANG Sheng-chun; HUANGFU Meng-jun; TIAN Wei-bin

doi:10.13422/j.cnki.syfjx.2010.05.067

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Analysis of Anti-hepatic Fibrosis Effects of WuLing Capsules Compatibility and Ingredients by Herbal with Drawal Analysis

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- Analysis of Anti-hepatic Fibrosis Effects of WuLing Capsules Compatibility and Ingredients by Herbal with Drawal Analysis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 5, Pages: 163-167(2010)
- 作者机构：
  
  第四军医大学西京医院药剂科
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.05.067
  CLC： R285.5
- Published：2010
- 稿件说明：
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[1]胡咏武,王胜春,皇甫孟军,田卫斌.撤药析方分析五灵胶囊配伍及组方成分抗肝纤维化作用[J].中国实验方剂学杂志,2010,16(05):163-167.

HU Yong-wu, WANG Sheng-chun, HUANGFU Meng-jun, et al. Analysis of Anti-hepatic Fibrosis Effects of WuLing Capsules Compatibility and Ingredients by Herbal with Drawal Analysis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 163-167.
[1]胡咏武,王胜春,皇甫孟军,田卫斌.撤药析方分析五灵胶囊配伍及组方成分抗肝纤维化作用[J].中国实验方剂学杂志,2010,16(05):163-167. DOI： 10.13422/j.cnki.syfjx.2010.05.067.

HU Yong-wu, WANG Sheng-chun, HUANGFU Meng-jun, et al. Analysis of Anti-hepatic Fibrosis Effects of WuLing Capsules Compatibility and Ingredients by Herbal with Drawal Analysis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 163-167. DOI： 10.13422/j.cnki.syfjx.2010.05.067.

摘要

目的:探讨五灵胶囊（Wuling Capsule

WL）组方成分和撤药组在方中的效应及对全方功效的影响。方法:采用四氯化碳多次注射

饮用10%乙醇溶液并以高脂低蛋白饲料饲养制备小鼠肝纤维化模型

分别给予试验药物治疗10d

酶法测定各实验组小鼠血清谷丙转氨酶（ALT）、胆碱酯酶（CHE）;体外培养大鼠肝星状细胞株HSC-T6细胞并采用转化生长因子（TGF-β1）诱导其活化

研究撤药组及组成成分对HSC-T6表达Ras/ERK

TGFβ/Smad信号通路蛋白的影响

Westernblot检测α-SMA

Ras

CollagenⅠ

ERK

p-ERK

TβRⅠ

TβRⅡ

Smad2/3蛋白的表达。结果:WL、组成成分和撤药组降低肝纤维化小鼠血清ALT和增加CHE活性

柴胡和灵芝无降ALT作用

各撤药组及组成成分对TGF-β1诱导HSC-T6表达α-SMA

CollagenⅠ

ERK

p-ERK

TβRⅠ

TβRⅡ

Smad2/3蛋白具有差异性生物学效应。结论:组方配伍对WL治疗纤维化小鼠作用产生相加的影响

抑制对TGF-b1诱导HSC-T6表达ERK和TGF-β/Smad信号通路蛋白表达

继而影响HSC-T6转录和合成COL-Ⅰ。

Abstract

Objective: To investigate the action of Wuling Capsules prescription ingredients and herbal withdrawal prescription and their effects on function of the original prescription. Method: The mouse model of hepatic fibrosis was established by multiple subcutaneous injection of carbon tetrachloride drinking 10% alcohol water solution

feeding hyperliposis-low protein diet and treated with experiment drugs for 10 days. The activities of ALT and CHE in serum of mice were detected by enzyme method. HSC-T6 cell was activated by transforming growth factor-beta1 in vitro

effects of the prescription ingredients and withdrawal drug compatibility on the expression of Ras /ERK and TGF-β /Smad signaling pathway pretein in HSC-T6 cell were observed. The expression of α-SMA

Ras

CollagenⅠ

ERK

p-ERK

TβRⅠ

TβRⅡ

Smad2 /3 were determined by western bloting. Result:The activity of ALT in surum of hepatic fibrisis mice was decreased by WuLing Capsules

prescription ingredients and withdrawal drug compatibility groups

while Bupleurum chinensis DC and Ganoderma group had no effects. WuLing Capsules

prescription ingrediets and withdrawal drug group had different biological effect on the protein expression of α-SMA

Collagen

ERK

p-ERK

TβRⅠ

TβRⅡ

Smad2 /3 in HSC-T6 cell induced by TGF-β1. Conclusion:Prescription ingredients compatibility produces additive effects on WuLing Capsules therapy for hepaticfibrosis mice

they inhibit the protein expression of ERK and TGF-β1 /Smad signaling pathway in HSC-T6 by TGF-β1 activation

subsequently influence the transcription and synthesis of collagenⅠin HSC-T6.

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HUANGFU Meng-jun

WANG Sheng-chun

HU Yong-wu

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HUANG Guidong

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Related Institution

Guangxi University of Chinese Medicine

Liuzhou People's Hospital， Guangxi Zhuang Autonomous Region Health Commission Guangxi Clinical Diseases Biotechnology Research Key Laboratory， Liuzhou Gastrointestinal Proprietary Chinese Medicine Engineering and Technology Research Center， Guangxi Medical University

Henan Institute of Traditional Chinese Medicine Diagnosis and Treatment Center First Affiliated Haspital Digestion

Hebei Provience Traditional Chinese Medicine Hospital

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