LI Peng, ZHANG Yu-yan, LI Jin-hui, et al. Preparation of Total Flavonoids of Flos Carthami and Radix Astragali from Compound Buyanghuanwu Decoction[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 10-13.
LI Peng, ZHANG Yu-yan, LI Jin-hui, et al. Preparation of Total Flavonoids of Flos Carthami and Radix Astragali from Compound Buyanghuanwu Decoction[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 10-13. DOI: 10.13422/j.cnki.syfjx.2010.06.005.
Preparation of Total Flavonoids of Flos Carthami and Radix Astragali from Compound Buyanghuanwu Decoction
摘要
目的:建立红花黄芪配伍总黄酮制备的最佳工艺。方法:采用水提醇沉法
以紫外分光光度法测定总黄酮含量
HPLC法测定羟基红花黄色素A(HYSA)的含量
对补阳还五汤中红花黄芪配伍总黄酮类的提取工艺进行系统研究
考察提取温度、提取时间、浸提料液比和浸提次数对提取效果的影响
用AB-8大孔吸附树脂对总黄酮部位进行精制纯化。结果:最佳工艺条件为:16倍量水
60℃
提取3次
每次2 h。AB-8树脂纯化后
黄酮纯度达78.26%
羟基红花黄色素A含量为10.07%。结论:该工艺简便
合理
对红花黄芪总黄酮有效部位的分离纯化较好。
Abstract
Objective:To establish the optimum preparation of total flavonoids from Flos Carthami and Ra-dix Astragali.Method:By water extraction and alcohol precipitation method
the optimal conditions
such as ex-traction temperature
extraction time
solvent quantity and extraction times were determined by orthogonal design.The total flavonoid was further prepared by AB-8 macroporous resin chromatography.Result:The optimum extrac-tion procedure was determined with 16 times of water
60 ℃
2 hours and 3 times.of extraction.The total fla-vonoids could reach to 78.6% and hydroxy safflor yellow A(HYSA) reach to 10.07% after further purification with AB-8 macroporous resin chromatography.Conclusion:This technique is suitable to prepare total flavonoids of Flos Carthami and Radix Astragali.