HPLC Analysis of Medicinal Serum of Radix Rehmanniae Preparata

CUI Ying1; WANG Xiao-ning; FANG Xiao-na1; DUAN Hong-fu1; DING Gang2

doi:10.13422/j.cnki.syfjx.2010.06.027

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HPLC Analysis of Medicinal Serum of Radix Rehmanniae Preparata

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- HPLC Analysis of Medicinal Serum of Radix Rehmanniae Preparata
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 6, Pages: 57-58(2010)
- 作者机构：
  
  1. 河南中医学院
  2. 江苏中康药物科技有限公司
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.06.027
  CLC： R285
- Published：2010
- 稿件说明：
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[1]崔瑛,王晓宁,房晓娜,段红福,丁岗.含熟地黄血清的高效液相色谱分析[J].中国实验方剂学杂志,2010,16(06):57-58+61.

CUI Ying1, WANG Xiao-ning, FANG Xiao-na1, et al. HPLC Analysis of Medicinal Serum of Radix Rehmanniae Preparata[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 57-58.
[1]崔瑛,王晓宁,房晓娜,段红福,丁岗.含熟地黄血清的高效液相色谱分析[J].中国实验方剂学杂志,2010,16(06):57-58+61. DOI： 10.13422/j.cnki.syfjx.2010.06.027.

CUI Ying1, WANG Xiao-ning, FANG Xiao-na1, et al. HPLC Analysis of Medicinal Serum of Radix Rehmanniae Preparata[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 57-58. DOI： 10.13422/j.cnki.syfjx.2010.06.027.

摘要

目的:探索熟地黄滋阴药效的物质基础对含熟地黄血清进行分析。方法:建立中医阴虚大鼠动物模型

用高效液相色谱法

通过比较熟地黄药材、阴虚大鼠含药血清、正常大鼠血清的高效液相指纹图谱

研究熟地黄血中移行成分。结果:含药血清中发现了1个与熟地黄原药材相同位置的波峰。结论:给药血清中与熟地黄药材相同位置的波峰

为熟地黄移行入血的原形成分

可能为熟地黄滋阴药效的活性成分

对其进行深入研究

有助于阐明熟地黄功效物质基础。

Abstract

Objective:The research aimed to analyse the medicinal serum of Prepared Radix Rehmanniae and explore the material base of Nourishing Yin effects on Prepared RadixRehmanniae.Method:Based on Yin-Deficiency Rats model

established HPLC fingerprints of Prepared Radix Rehmannia

The constituents absorbed into blood were determined by comparing HPLC fingerprints of Prepared Radix Rehmanniae

medicinal serum of Yin deficiency rats and blank serum.Result:After oral administration

one Chromatographic Peak was found in the medicinal serum as the same location as Prepared Radix Rehmanniae.Conclusion:The Chromatographic Peak in the medicinal serum which is as the same location as Prepared Radix Rehmanniae is the original constituent of Prepared Radix Rehmanniae absorbed into blood.and might be active component of nourishing yin.Further studies of them is helpful to clarify the bioactive material Base of Prepared Radix Rehmanniae.

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Related Author

CUI Ying

WANG Xiao-ning

FANG Xiao-na

DUAN Hong-fu

DING Gang

Hong-xing MA

Long-fei LIN

Yu-ling LIU

Related Institution

Jilin Agricultural University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

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Jilin Huakang Pharmaceutical Co. Ltd.

The First Clinical Medical College of Guangzhou University of Chinese Medicine

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