Protective Effects and Mechanism of Danju Capsule on Angiotensin Ⅱ-Induced Dndothelial Dysfunction

YI Jia-li1; ZHANG Zhong-ai2; LUO Tian-jiong2; LIU Bao-lin1

doi:10.13422/j.cnki.syfjx.2010.06.035

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Protective Effects and Mechanism of Danju Capsule on Angiotensin Ⅱ-Induced Dndothelial Dysfunction

更新时间：2024-09-23

- Protective Effects and Mechanism of Danju Capsule on Angiotensin Ⅱ-Induced Dndothelial Dysfunction
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 6, Pages: 202-206(2010)
- 作者机构：
  
  1. 中国药科大学中药药理学教研室
  2. 南京市中医院老年病科
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.06.035
  CLC： R285.5
- Published：2010
- 稿件说明：
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[1]易佳丽,张钟爱,骆天炯,刘保林.丹桔胶囊对血管紧张素Ⅱ损伤血管内皮功能的保护作用[J].中国实验方剂学杂志,2010,16(06):202-206.

YI Jia-li1, ZHANG Zhong-ai2, LUO Tian-jiong2, et al. Protective Effects and Mechanism of Danju Capsule on Angiotensin Ⅱ-Induced Dndothelial Dysfunction[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 202-206.
[1]易佳丽,张钟爱,骆天炯,刘保林.丹桔胶囊对血管紧张素Ⅱ损伤血管内皮功能的保护作用[J].中国实验方剂学杂志,2010,16(06):202-206. DOI： 10.13422/j.cnki.syfjx.2010.06.035.

YI Jia-li1, ZHANG Zhong-ai2, LUO Tian-jiong2, et al. Protective Effects and Mechanism of Danju Capsule on Angiotensin Ⅱ-Induced Dndothelial Dysfunction[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 202-206. DOI： 10.13422/j.cnki.syfjx.2010.06.035.

摘要

目的:观察丹桔胶囊对血管紧张素Ⅱ（AngⅡ）损伤血管内皮功能的保护作用。方法:制备大鼠胸主动脉环

观察丹桔胶囊提取剂对抗AngⅡ引起的胸主动脉环收缩反应;AngⅡ刺激人脐静脉血管内皮细胞（HUVEC）以反转录—聚合酶链反应（RT-PCR）方法检测HUVEC的内皮素（ET-1）mRNA表达水平;蛋白质印迹（Western blot）方法检测HUVEC中细胞外信号调节激酶（extracellular signal-regulated kinase

ERK）和核转录因子κB（nuclear factorκB

NF-κB）的活化情况。结果:AngⅡ引起胸主动脉收缩

丹桔胶囊制剂能显著对抗血管收缩作用

下调AngⅡ刺激所致的HUVEC细胞ET-1 mRNA过度表达

抑制ERK1/2磷酸化

同时对NF-的活化表现出显著的抑制作用。p65位移、IκB-α磷酸化降解

丹桔胶囊上述抑制作用均呈现浓度依赖性。结论:丹桔胶囊抑制AngⅡ对血管内皮功能的损伤

其机制与降低ET-1表达

抑制ERK/NF-κB途径活化有关。

Abstract

Objective:To observe the protective effect of Danju Capsule on vascular endothelial dysfunction induced by angiotensinⅡ（AngⅡ）.Method:The Ang Ⅱ-induced contraction of rat aortic rings was tested in tissue bath.The expression of endothelin（ET）-1 mRNA was evaluated by RT-PCR in human umbilical vein endothelial cells（HUVEC） stimulated by Ang II.The activation of extracellular signal-regulated kinase（ERK） and nuclear transcription factor（NF-κB） and its inhibitor（IκB-α） were detected by Western blot in HUVEC.Result:The results showed that 1 × 10-7 mol.L-1 Ang Ⅱ increased contraction of rat aortic rings

ET-1mRNA expression

ERK1 /2 phosphorylation

NF-κB p65 displacement and IκB-α phosphorylation degradation

which were significantly inhibited by Danju Capsule in a dose-dependent manner.Conclusion:Ang Ⅱ may be involved in the initiation and progression of cardiovascular diseases by injuring vascular endothelium directly and causing endothelial dysfunction.Danju Capsule was shown to protect against Ang Ⅱ induced endothelial dysfunction

suggesting that Danju Capsule may play a role in the prevention and treatment of cardiovascular diseases.

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Related Author

YI Jia-li

ZHANG Zhong-ai

LUO Tian-jiong

LIU Bao-lin

ZHANG Yuanyuan

JIN Peipei

BIAN Dong

GUO Dengzhou

Related Institution

Graduate School， Hebei University of Chinese Medicine

The First Affiliated Hospital of Hebei University of Chinese Medicine/Hebei Provincial Hospital of Traditional Chinese Medicine

Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine

Key Laboratory of Ministry of Education for Traditional Chinese Medicine (TCM) Viscera-State Theory and Applications, Liaoning University of TCM

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

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