ZHANG Jin-lian, HE Min, XIE Yi-hui, et al. Determination of Content of Narigin and Hesperidin and Neohesperidin in Fructus Auranti Pieces by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 68-70.
ZHANG Jin-lian, HE Min, XIE Yi-hui, et al. Determination of Content of Narigin and Hesperidin and Neohesperidin in Fructus Auranti Pieces by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 68-70. DOI: 10.13422/j.cnki.syfjx.2010.06.048.
Determination of Content of Narigin and Hesperidin and Neohesperidin in Fructus Auranti Pieces by HPLC
摘要
目的:建立高效液相法测定枳壳饮片中柚皮苷、橙皮苷和新橙皮苷定量分析方法。方法:Ultimate 3000 Cl8色谱柱(4.6 mm×250 mm
Objective:To establish the method of determining the content of narigin and hesperidin and neohesperidin in Fructus Auranti pieces by HPLC.Method:Ultimate 3 000 Cl8 column(4.6 mm × 250 mm
5 μm)
mobile phase was acetonitrile-water(pH was adjusted to 3 by phosphoric acid)(23∶77).Wavelength was 283 nm
flow rate was 1.0 mL.min-1
column temperature was 30 ℃.Result:naringin
hesperidin and neohesperidin linear ranges were 0.316 4 ~ 1.582 4 μg(r = 0.999 9)
0.041 2 ~ 0.206 0 μg(r = 0.999 9)
0.188 0 ~ 0.940 0 μg(r = 0.999 9).The average coefficient of recovery were greater than 96.11%.Conclusion:This method is highly specific
accurate and reliable and has good repeatability.It is suitable for the determination of the content of narigin and hesperidin and neohesperidin in Fructus Auranti pieces.It can be widely used for the quality control of Fructus Auranti pieces.
Determination of Content of Narigin and Hesperidin and Neohesperidin in Fructus Auranti Pieces by HPLC
Simultaneous Determination of 5 Active Ingredients in Jinggu Tablets by HPLC Dual-wavelength Detection
HPLC Determination of Four Flavonoids of Aurantii Fructus Immaturus in Niuhuang Qingwei Pills and Genetic Analysis of Medicinal Materials
Determination of Flavone Ingredients in Citrus Aurantium, Bitter Orange Immature, Walnut Peel and Tangerine Peel By Internal Standard Multi-control Method
HPLC Determination of Naringin, Hesperidin and Neohesperidin in Citri Reticulatae Pericarpium and Aurantii Fructus Extract
Related Author
ZHANG Jin-lian
HE Min
XIE Yi-hui
YAO Dong-qin
YU Run-min
ZHANG Di-feng
ZENG Qi
TAN Dao-peng
Related Institution
National Institutes for Food and Drug Control
The National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine
Jiangxi University of Traditional Chinese Medicine
Jiangxi University of Chinese Medicine,Research Center for Traditional Chinese Medicine Resources and Ethnic Minority Medicine