ZHU Xiang-yu1, YANG Lian-ju2, LI Chun2. Determination of Protocatechuic Acid in Trogopterus xanthippes Milne-edwards by High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 71-73.
ZHU Xiang-yu1, YANG Lian-ju2, LI Chun2. Determination of Protocatechuic Acid in Trogopterus xanthippes Milne-edwards by High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 71-73. DOI: 10.13422/j.cnki.syfjx.2010.06.049.
Determination of Protocatechuic Acid in Trogopterus xanthippes Milne-edwards by High Performance Liquid Chromatography
Objective:A method was developed for the quantitative determination of protocatechuic acid in Trogopterus xanthippes Milne-Edwards by HPLC.Method: The analyst was determined by HPLC with a Hypersil GOLD C18 column and a mobile phase of menthol-1% acetic acid-water(5∶ 95)
at a flow rate of 1 mL.min-1.The UV detection wave length was 260 nm.Result: The assay exhibited a good linearity in the concentration range from 0.019 9 to 0.099 5 μg with a correlation coefficient of 0.999 9.The analyst ’s precision
stabilization
reappearance and recovery were all in limited range.Conclusion:This method was reliable and could be used for the quality control of Trogopterus xanthippes Milne-Edwards.