Simultaneous Determination of Brucine and Strychnine in Rat Plasma by HPLC

XIAO Han-lu1; CHEN Jun1; CAI Bao-chang1; HOU Ting1; FANG Yun2

doi:10.13422/j.cnki.syfjx.2010.11.017

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Simultaneous Determination of Brucine and Strychnine in Rat Plasma by HPLC

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- Simultaneous Determination of Brucine and Strychnine in Rat Plasma by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 11, Pages: 26-29(2010)
- 作者机构：
  
  1. 南京中医药大学药学院
  2. 南京大学医学院附属鼓楼医院药剂科
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.11.017
  CLC： R285
- Published：2010
- 稿件说明：
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[1]肖寒露,陈军,蔡宝昌,侯婷,方芸.HPLC同时测定大鼠血浆中马钱子碱和士的宁的浓度[J].中国实验方剂学杂志,2010,16(11):26-29.

XIAO Han-lu1, CHEN Jun1, CAI Bao-chang1, et al. Simultaneous Determination of Brucine and Strychnine in Rat Plasma by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 26-29.
[1]肖寒露,陈军,蔡宝昌,侯婷,方芸.HPLC同时测定大鼠血浆中马钱子碱和士的宁的浓度[J].中国实验方剂学杂志,2010,16(11):26-29. DOI： 10.13422/j.cnki.syfjx.2010.11.017.

XIAO Han-lu1, CHEN Jun1, CAI Bao-chang1, et al. Simultaneous Determination of Brucine and Strychnine in Rat Plasma by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 26-29. DOI： 10.13422/j.cnki.syfjx.2010.11.017.

摘要

目的:建立能同时测定大鼠血浆中马钱子碱和士的宁浓度的HPLC分析方法。方法:大鼠血浆样品以石杉碱甲为内标

液液萃取法处理后用Kromasil C18柱分离

以乙腈-0.01 mol.L-1庚烷磺酸钠与0.02 mol.L-1磷酸二氢钾等量混合后用10%磷酸调体系pH 2.8（24∶76）作流动相

检测波长264 nm。结果:血浆中杂质不干扰样品的含量测定

马钱子碱和士的宁在50～2 000μg.L-1线性良好

平均提取回收率均大于86%

日内、日间精密度均小于8%

稳定性符合体内药物分析要求。结论:方法灵敏度高、精密度好

可应用于马钱子总生物碱中主要成分的药物动力学研究。

Abstract

Objective:To establish an effective method for simultaneously determining the concentration of brucine and strychnine in rat plasma by HPLC.Method:The processed rat plasma samples were separated on a Kromasil C18 column.The mobile phase consisted of acetonitrile-0.01 mol.L-1 sodium heptane sulfonate and 0.02 mol.L-1 potassium dihydrogen phosphate

adjusted pH to 2.8 with 10% phosphonic acid（24∶ 76）.The detection wavelength was set at 264 nm.Result:Chromatograms showed endogenous peaks not interfering with the analysis.The calibration curves of brucine and strychnine were both in good linearity within the range of 50-2 000 μg.L-1.The mean extraction recoveries were more than 86% while the precisions were less than 8%.The results of stability experiment meet certain requirements for the biopharmaceutical analysis.Conclusion:The method with good sensitivity and precision is applicable to the pharmacokinetic studies of the main compounds in total alkaloids from seeds of Strychnou nux-vomica.

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Related Author

XIAO Han-lu

Chen Jun

CAI Bao-chang

HOU Ting

FANG Yun

ZHANG Xiao-jun

JIN Ri-xian

CHEN Yan-jun

Related Institution

Institute of Cinese Materia Medica,China Academy of Chinese Medical Scineces

中国中医科学院中药研究所北京100700

Jilin Agricultural University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Chengde Medical University

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