ZHOU Zheng-li1, LI Feng1, HU Ping2. GC/MS Fingerprints and Fisher Discrimination Analysis on Water-soluble Sugar from 20 Chinese Herbs with Cold and Heat Properties[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 41-44.
ZHOU Zheng-li1, LI Feng1, HU Ping2. GC/MS Fingerprints and Fisher Discrimination Analysis on Water-soluble Sugar from 20 Chinese Herbs with Cold and Heat Properties[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 41-44. DOI: 10.13422/j.cnki.syfjx.2010.11.022.
GC/MS Fingerprints and Fisher Discrimination Analysis on Water-soluble Sugar from 20 Chinese Herbs with Cold and Heat Properties
摘要
目的:探讨中药寒热药性与水溶性糖的相关性
进一步阐明中药四性与物质基础的关系。方法:选取10种寒性中药和10种热性中药
提取水溶性糖
分别加入盐酸羟胺、吡啶和乙酸酐进行乙酰化反应
测定水溶性糖的GC/MS指纹图谱
利用Fisher方法建立判别函数
分析实验结果。结果:Fisher判别函数实现了对20种中药寒热药性的准确识别
组内回代一致率为100%。结论:Fisher可以作为判别中药寒热药性与水溶性糖关系的统计学工具
水溶性糖是中药寒热药性的物质基础之一。
Abstract
Objecive:To discuss the relationship between water-soluble sugar and cold-heat properties and further reveal correlations of four properties and material basis.Method:Water-soluble sugars were purified from 10 herbs with cold properties and 10 herbs with heat properties.After acetylation
GC /MS fingerprints of water-soluble sugars were determined and the results were analyzed by Fisher.Result:Discriminant function had been established by Fisher.The Fisher discriminant analysis could distinguish the cold and heat properties between 20 herbs that the back substitution in group was 100%.There was difference between the drugs with cold and heat properties in the GC /MS fingerprints
which corresponded to the different water-soluble sugars in the cold and heat herbs.Conclusion:The Fisher analysis could differentiate the herbs with cold and heat properties
and it was an effective statistical tool to study the relationship between the cold and heat properties by water-soluble sugar.It concludes that the water-soluble sugar is one of the material basis of four properties of herbs.