Determination of Euphol in Slices of Euphorbia kansui and Kongxian Pills by HPLC-ELSD

XIU Yan-feng1; SHI Bei1; ZHU Cheng1

doi:10.13422/j.cnki.syfjx.2010.11.028

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Determination of Euphol in Slices of Euphorbia kansui and Kongxian Pills by HPLC-ELSD

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- Determination of Euphol in Slices of Euphorbia kansui and Kongxian Pills by HPLC-ELSD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 11, Pages: 61-63(2010)
- 作者机构：
  
  1. 上海中医药大学中药学院
  2. 南京中医药大学江苏省中药炮制重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.11.028
  CLC： R286;R284.1
- Published：2010
- 稿件说明：
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[1]修彦凤,施贝,朱程.HPLC-ELSD测定甘遂及其制剂控涎丸中大戟二烯醇的含量[J].中国实验方剂学杂志,2010,16(11):61-63.

XIU Yan-feng1, SHI Bei1, ZHU Cheng1. Determination of Euphol in Slices of Euphorbia kansui and Kongxian Pills by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 61-63.
[1]修彦凤,施贝,朱程.HPLC-ELSD测定甘遂及其制剂控涎丸中大戟二烯醇的含量[J].中国实验方剂学杂志,2010,16(11):61-63. DOI： 10.13422/j.cnki.syfjx.2010.11.028.

XIU Yan-feng1, SHI Bei1, ZHU Cheng1. Determination of Euphol in Slices of Euphorbia kansui and Kongxian Pills by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 61-63. DOI： 10.13422/j.cnki.syfjx.2010.11.028.

摘要

目的:建立甘遂及其制剂控涎丸中大戟二烯醇的含量测定方法。方法:色谱柱为Dikma Diamonsil C18柱（4.6 mm×250 mm

5μm）

流动相甲醇-水（99∶1）

流速1.0 mL.min-1

检测器蒸发光散射检测器

柱温35℃。结果:大戟二烯醇在进样量为1.04～20.80μg（r=0.999 6）

进样量的常用对数值与峰面积的常用对数值呈良好的线性关系

平均回收率97.53%

RSD 2.56%

另外对10批甘遂饮片和5批控涎丸进行了测定。结论:所建立的方法简单、准确、重复性好

可用于测定甘遂及其制剂控涎丸中大戟二烯醇的含量。

Abstract

Objective:To establish a HPLC method for the content determination of euphol in slices of E.kansui and its preparation Kongxian Pills.Method:HPLC-ELSD was carried out on a Dikma Diamonsil C18 column（4.6 mm × 250 mm

5 μm）with methanol-water（99∶ 1） as the mobile phase

the flow rate was 1.0 mL.min-1

and the detector was evaporative light-scattering detector（ELSD）.The column temperature was set at 35 ℃.Result:The calibration curve was linear in the range of 1.04-20.80 μg（r = 0.999 6）

the average recovery was 97.53% with RSD of 2.56%.The content of euphol in ten batches of slices of E.kansui and five batches of Kongxian Pills were determined.Conclusion:The method is simple

accurate and reproducible

and it can be used to determine the content of euphol in slices of E.kansui and Kongxian Pills.

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XIU Yan-feng

SHI Bei

ZHU Cheng

ZHANG Xu-qian

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DENG Chao-ming

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Related Institution

Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine（TCM），School of Pharmacy，Henan University of Chinese Medicine

Luohe Medical College

Third Affiliated Hospital of Henan University of Chinese Medicine

Tianjin University of Traditional Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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