Determination of Quercitrin from Platycladus orientalis Leaves by Capillary-zone Electrophoresis

DING Hong-mei; SHENG Zhen-hua; GE Er-ning

doi:10.13422/j.cnki.syfjx.2010.11.030

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Determination of Quercitrin from Platycladus orientalis Leaves by Capillary-zone Electrophoresis

更新时间：2024-09-23

- Determination of Quercitrin from Platycladus orientalis Leaves by Capillary-zone Electrophoresis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 11, Pages: 73-75(2010)
- 作者机构：
  
  浙江中医药大学分析测试中心
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.11.030
  CLC： R284.1
- Published：2010
- 稿件说明：
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[1]丁红梅,盛振华,葛尔宁.毛细管区带电泳法测定侧柏叶中槲皮苷含量[J].中国实验方剂学杂志,2010,16(11):73-75.

DING Hong-mei, SHENG Zhen-hua, GE Er-ning. Determination of Quercitrin from Platycladus orientalis Leaves by Capillary-zone Electrophoresis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 73-75.
[1]丁红梅,盛振华,葛尔宁.毛细管区带电泳法测定侧柏叶中槲皮苷含量[J].中国实验方剂学杂志,2010,16(11):73-75. DOI： 10.13422/j.cnki.syfjx.2010.11.030.

DING Hong-mei, SHENG Zhen-hua, GE Er-ning. Determination of Quercitrin from Platycladus orientalis Leaves by Capillary-zone Electrophoresis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 73-75. DOI： 10.13422/j.cnki.syfjx.2010.11.030.

摘要

目的:建立毛细管区带电泳（CZE）分离测定中药侧柏叶中槲皮苷的新方法。方法:以30 mmol.L-1硼砂-硼酸（体积比1∶3）为运行缓冲液

分离电压25 kV

温度25℃

压力进样0.5 psi×8 s

运行时间7 min为电泳条件

在254 nm波长下检测侧柏叶中的槲皮苷。结果:槲皮苷在1～200 mg.L-1线性关系良好

精密度、稳定性、加样回收率RSD均小于3%

测得3个批次侧柏叶中槲皮苷含量分别为2.551 3

2.546 7

2.478 0 mg.g-1。结论:该方法简便、准确

为此类药物的质量控制提供了一种快速的检测方法。

Abstract

Objective:To establish a new determination method for quercitrin from Platycladus orientalis leaves by capillary-zone electrophoresis（CZE）.Method:Under the optimized CZE conditions:a running buffer composed of 30 mmol.L-1 borax-boric acid（volume 1∶ 3）

separation voltage at 25 kV

temperature at 25 centigrade

injection time for 8 sec with 0.5 psi pressure

quercitrin from P.orientalis leaves was detected at 254 nm wavelength.Result:Quercitrin was linear in the range of 1-200 mg.L-1（r = 0.999 3）.The RSD of exactitude

stability

and recovery of the method was 1.1346%

2.6046 % and 2.3315%

respectively.The contents of quercitrin in three batches of P.orientalis leaves were 2.551 3

2.546 7

2.478 0 mg.g-1.Conclusion:The method was simple

fast and accurate for determination of the drugs containing quercitrin.

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Related Author

DING Hong-mei

SHENG Zhen-hua

GE Er-ning

WEI Gang1

HUANG Yue-chun2

CHEN Mu-yuan1

LIU Dong-hui1

CHEN Mu-yuan2

Related Institution

Deptartment of New Drug R&D in Guangzhou University of Chinese Medicine

Dongguan Institution for Mathematics and Theoretics Engineering Research

The Frist Affiliated Hospital of Guangzhou University of TCM

Guangzhou University of TCM

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