ZHENG Li-xiang, LIU Hong-ning, QIAO Yu-dan, et al. Effect of Liuwei Dihuang Wan on Vascular Endothelial Grouth Factor Expression and Cyclin D3 Gene in Spontaneous Breast Carcinoma in Mice[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 117-119.
ZHENG Li-xiang, LIU Hong-ning, QIAO Yu-dan, et al. Effect of Liuwei Dihuang Wan on Vascular Endothelial Grouth Factor Expression and Cyclin D3 Gene in Spontaneous Breast Carcinoma in Mice[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 117-119. DOI: 10.13422/j.cnki.syfjx.2010.11.040.
Effect of Liuwei Dihuang Wan on Vascular Endothelial Grouth Factor Expression and Cyclin D3 Gene in Spontaneous Breast Carcinoma in Mice
Objective:To investigate the effect of Liuwei Dihuang Wan on spontaneous breast carcinoma in mice.Method:The spontaneous breast carcinoma derived in kunming mice(clean class) was used as animal model.The mice were given Liuwei Dihuang Wan to their agonal stage.The cancer mass pathology was analyzed by HE staining.The mRNA and protein expressions of vascular endothelial growth factor(VEGF) and cyclin D3 were assayed by RT-PCR and Western blotting.Result:In Liuwei Dihuang Wan group
the tumor weight and expression of VEGF and cyclin D3 gene were obviously lower than that in the control groups(P < 0.05).The cell differentiation was also better than that in the control group.Conclusion:Liuwei Dihuang Wan can retrain the growth of cancer and promote the cancer cell differentiation.The possible mechanism is related with the retrained expression of VEGF and cyclin D3 gene.
Effect of Liuwei Dihuang Wan on Vascular Endothelial Grouth Factor Expression and Cyclin D3 Gene in Spontaneous Breast Carcinoma in Mice
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Related Author
ZHENG Li-xiang
LIU Hong-yu
QIAO Hong-dan
Liu Xia
LIN Dong-mei
LIU Quan
HE Yiqing
ZHANG Ying
Related Institution
Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine
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