Effects of Wutian Baogan Ye on Peroxisome Proliferator-activated Receptor γ Expression for Alcohol Hepatic Fibrosis in Rats

SU Jin-ling1; BI Ye-dong2; YU Jian-jiang2; JIANG Xi-juan1; SONG Xing-xing1; FAN Ying-chang1

doi:10.13422/j.cnki.syfjx.2010.11.045

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Effects of Wutian Baogan Ye on Peroxisome Proliferator-activated Receptor γ Expression for Alcohol Hepatic Fibrosis in Rats

更新时间：2024-09-23

- Effects of Wutian Baogan Ye on Peroxisome Proliferator-activated Receptor γ Expression for Alcohol Hepatic Fibrosis in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 11, Pages: 139-142(2010)
- 作者机构：
  
  1. 天津中医药大学病理教研室
  2. 天津市汉沽区中医医院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.11.045
  CLC： R285.5
- Published：2010
- 稿件说明：
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[1]苏金玲,毕业东,于建江,姜希娟,宋星星,范英昌.五田保肝液对酒精性肝纤维化大鼠肝脏过氧化物酶体增殖物激活受体γ表达的影响[J].中国实验方剂学杂志,2010,16(11):139-142+144.

SU Jin-ling1, BI Ye-dong2, YU Jian-jiang2, et al. Effects of Wutian Baogan Ye on Peroxisome Proliferator-activated Receptor γ Expression for Alcohol Hepatic Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 139-142.
[1]苏金玲,毕业东,于建江,姜希娟,宋星星,范英昌.五田保肝液对酒精性肝纤维化大鼠肝脏过氧化物酶体增殖物激活受体γ表达的影响[J].中国实验方剂学杂志,2010,16(11):139-142+144. DOI： 10.13422/j.cnki.syfjx.2010.11.045.

SU Jin-ling1, BI Ye-dong2, YU Jian-jiang2, et al. Effects of Wutian Baogan Ye on Peroxisome Proliferator-activated Receptor γ Expression for Alcohol Hepatic Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 139-142. DOI： 10.13422/j.cnki.syfjx.2010.11.045.

摘要

目的:探讨五田保肝液对大鼠酒精性肝纤维化的干预作用及对酒精性肝纤维化大鼠肝脏过氧化物酶体增殖物激活受体γ（PPARγ）表达的影响。方法:将100只Wistar大鼠随机分成6组:正常组10只、模型组、五田保肝液低、中、高剂量组、易善复组各18只

采用白酒辅以吡唑、玉米油的混合食料ig的方法建立大鼠酒精性肝纤维化模型

12周末处死大鼠

HE染色观察肝组织形态学改变;Masson染色观察肝纤维化情况;酶联免疫吸附法（ELISA）检测大鼠血清层黏连蛋白（LN）

Ⅲ型前胶原（PⅢP）的水平变化;免疫组化法检测大鼠肝组织α-平滑肌肌动蛋白（α-SMA）蛋白的表达;实时定量RT-PCR法检测肝组织PPARγmRNA的表达。结果:与模型组相比

五田保肝液和易善复可减轻大鼠肝脏胶原纤维的增生

减少α-SMA蛋白的表达

使大鼠血清LN和PⅢP水平下降

肝组织PPARγmRNA的表达升高

且以五田保肝液中、高剂量效果更显著。PPARγmRNA的表达与α-SMA蛋白表达呈负相关。结论:五田保肝液对酒精性肝纤维化大鼠肝组织PPARγ表达具有促进作用

这可能是五田保肝液抑制大鼠酒精性肝纤维化发生发展的机制之一。

Abstract

Objective:To investigate effects of Wutian Baogan Ye on alcohol hepatic fibrosis in rats.Meth-od:One hundred Wistar rats were divided randomly into six groups:a normal group（10）

a model control group（18）

Wutian Baogan Ye low dose group（18）

middle dose group（18）

high dose group（18）

and Yishanfu group（18）.Rat model of alcohol hepatic fibrosis was induced by perfusing stomach with the mixture of alcohol

pyrazol and corn oil.After 12 weeks intervention

the indexes of hepatic fiborsis and expression of α-SMA

PPARγ mRNA in liver tissue were investigated for rats in different groups.Result:Compared with model rats

Wutian Ban-gan Ye（especially the middle and the high dose） and Yishanfu could suppress the development of fibrosis

the lev-els of PⅢP

LN in serum and the expression of α-SMA

and increase the expression of PPAR-γ mRNA.The ex-pression of α-SMA and PPARγ was negatively correlated.Conclusion:Wutian Baogan Ye has an active effect on inhibition of alcohol hepatic fibrosis in rats

with a possible mechanism of promoting the expression of PPARγ.

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湖北中医学院附属医院神经内科

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