DPPH and ABTS Radical Scavenging Capacity of Elsholtzia rugulosa

GUO Zhi-qin1; LV Hai-ning1; CHEN Qiao-lian2; SHE Gai-mei1

doi:10.13422/j.cnki.syfjx.2010.11.057

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DPPH and ABTS Radical Scavenging Capacity of Elsholtzia rugulosa

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- DPPH and ABTS Radical Scavenging Capacity of Elsholtzia rugulosa
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 11, Pages: 180-183(2010)
- 作者机构：
  
  1. 北京中医药大学中药学院中药化学系
  2. 中国农业大学研究生院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.11.057
  CLC： R285.5
- Published：2010
- 稿件说明：
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[1]郭志琴,吕海宁,陈巧莲,折改梅.野坝子体外清除自由基活性研究[J].中国实验方剂学杂志,2010,16(11):180-183.

GUO Zhi-qin1, LV Hai-ning1, CHEN Qiao-lian2, et al. DPPH and ABTS Radical Scavenging Capacity of Elsholtzia rugulosa[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 180-183.
[1]郭志琴,吕海宁,陈巧莲,折改梅.野坝子体外清除自由基活性研究[J].中国实验方剂学杂志,2010,16(11):180-183. DOI： 10.13422/j.cnki.syfjx.2010.11.057.

GUO Zhi-qin1, LV Hai-ning1, CHEN Qiao-lian2, et al. DPPH and ABTS Radical Scavenging Capacity of Elsholtzia rugulosa[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 180-183. DOI： 10.13422/j.cnki.syfjx.2010.11.057.

摘要

目的:对野坝子Elsholtzia rugulosa Hemsl.乙醇提取物的不同极性萃取部位和AB-8大孔树脂柱色谱不同浓度甲醇洗脱部位清除自由基能力进行综合评价。方法:分别以维生素C和维生素E为对照

采用清除2

2-二苯基苦味酰基苯肼基（2

2-diphenyl-1-picrylhydrazyl

DPPH）和2

2′-连氮基-双-（3-乙基苯并噻唑啉-6-磺酸）二铵盐[2

2′-azino-bis（3-ethylbenzthiazo-line-6-sulphonic acid）diammonium salt

ABTS]自由基的方法

通过计算半数抑制浓度评价野坝子的乙醇提取物的不同极性萃取部位和AB-8大孔树脂柱色谱不同浓度甲醇洗脱部位的清除自由基能力。结果:野坝子提取物不同极性萃取部位清除DPPH自由基和ABTS自由基的能力分别为:乙酸乙酯部位>Vit C>正丁醇部位>水部位>石油醚部位>氯仿部位、乙酸乙酯部位>Vit E>正丁醇部位>水部位>氯仿部位>石油醚部位。AB-8大孔树脂柱色谱不同浓度甲醇洗脱部位清除DPPH和ABTS自由基的能力分别为Vit C>50%甲醇部位>70%甲醇部位>30%甲醇部位>100%甲醇部位、50%甲醇部位>70%甲醇部位>30%甲醇部位>Vit E>100%甲醇部位。结论:野坝子乙醇提取物的乙酸乙酯萃取部位和30%

50%和70%甲醇洗脱部位都具有很好的清除自由基活性

是一种新型天然抗氧化物质来源。

Abstract

The free radical scavenging capacity of different extracts and macroporous resin column chroma-tography gradient solutions from ethanol extract of Elsholtzia rugulosa were determined by 2

2-diphenyl-1-picryl-hydrazyl（DPPH） and improved 2

2′-azino-bis（3-ethylbenzthiazoline-6-sulphonic acid） diammonium salt（ABTS） assay

with Vit C and Vit E as the control.The free radical scavenging capacity on DPPH and ABTS of different ex-tracts of E.rugulosa followed by ethyl acetate > Vit C > n-butanol > aqueous solution > petroleum ether > chloro-form and ethyl acetate > Vit E > n-butanol > aqueous solution > chloroform > petroleum ether

respectively.The free radical scavenging capacity on DPPH and ABTS of macroporous resin column chromatography gradient solutions were Vit C > 50% MeOH > 70% MeOH > 30% MeOH > 100% MeOH and 50% MeOH > 70% MeOH > 30% MeOH > Vit E > 100% MeOH.The ethyl acetate extracts and 30%

50% and 70% MeOH solutions exhibited strong antiox-idant activity.It indicated as a new source of natural antioxidants.

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DPPH and ABTS Radical Scavenging Capacity of Elsholtzia rugulosa

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