To Determine Content of Total Flavonoids and Robinin-7-o-β-D-xylosylglucoside from Dracoephalum mololarie

SONG Rui1; JIN Chuan-shan1; ZHOU Ya-wei2

doi:10.13422/j.cnki.syfjx.2010.12.023

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To Determine Content of Total Flavonoids and Robinin-7-o-β-D-xylosylglucoside from Dracoephalum mololarie

更新时间：2024-09-23

- To Determine Content of Total Flavonoids and Robinin-7-o-β-D-xylosylglucoside from Dracoephalum mololarie
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 12, Pages: 71-74(2010)
- 作者机构：
  
  1. 安徽中医学院药学院
  2. 北大世佳研究中心
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2010.12.023
  CLC： R284.1
- Published：2010
- 稿件说明：
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[1]宋睿,金传山,周亚伟.香青兰中总黄酮和单体的含量测定[J].中国实验方剂学杂志,2010,16(12):71-74.

SONG Rui1, JIN Chuan-shan1, ZHOU Ya-wei2. To Determine Content of Total Flavonoids and Robinin-7-o-β-D-xylosylglucoside from Dracoephalum mololarie[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 71-74.
[1]宋睿,金传山,周亚伟.香青兰中总黄酮和单体的含量测定[J].中国实验方剂学杂志,2010,16(12):71-74. DOI： 10.13422/j.cnki.syfjx.2010.12.023.

SONG Rui1, JIN Chuan-shan1, ZHOU Ya-wei2. To Determine Content of Total Flavonoids and Robinin-7-o-β-D-xylosylglucoside from Dracoephalum mololarie[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(12): 71-74. DOI： 10.13422/j.cnki.syfjx.2010.12.023.

摘要

目的:考察香青兰中单体（刺槐素-7-o-β-D-葡萄糖苷）、总黄酮的含量。方法:采用紫外分光光度法（UV）测定总黄酮含量;采用高效液相色谱法（HPLC）测定单体含量

RP-C18柱（4.6 mm×250 mm

5μm）

流动相为乙腈-0.1%磷酸水（26∶74）

流速为1.1 mL·min-1

柱温室温

检测波长为326 nm。结果:单体（刺槐素-7-o-β-D-葡萄糖苷）含量为0.33%

回收率为98.62%;总黄酮含量为1.90%

回收率为97.59%。结论:所建方法准确、简便

为科学地评价香青兰药材的质量提供依据。

Abstract

Objective: To determine the content of total flavonoids and robinin-7-o-β-D-xylosylglucoside fron Dracoephalum mololarie.Method: The content of total flavonoids was determined by ultraviolet spectrophotometry（UV）.The content of robinin-7-o-β-D-xylosylglucoside was determined by HPLC.The analysis was performed on a RP-C18 column（4.6 mm × 250 mm

5 μm） with aceconitrile-0.1% phosphoric acid（26∶ 74） as mobile phase at a flow rate of 1.1 mL.min-1

and at room temperature.The detection of wave length was set at 326 nm.Result: The content of robinin-7-o-β-D-xylosylglucoside was 0.33%

and recovery rate was 98.62%.The content of total flavonoids was 1.90%

and recovery rate was 97.59%.Conclusion: The method is suitable for the determination of flavonoids components from D.mololariea.

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ZHOU Ya-wei

LI Ming-jiang

ZHANG Yu-ling

PAN Yang

ZHANG Xian

SUN Hong

Related Institution

Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences and Peking Union Medical College

School of Life Sciences， Tsinghua University

Center of Pharmacy R&D，XinJiang Huachun Bio-Pharmaceutical Co. Ltd.

Pharmacy Technology Center，Tsinghua University

School of Pharmacy， Henan University of Chinese Medicine

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